Mouse anti-USP14 Monoclonal Antibody(N-term)(889CT6.2.1)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
USP14抗体;Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14; USP14; TGT
|
| 宿主 |
Mouse
|
| 特异性 |
Purified His-tagged USP14 protein was used to produced this monoclonal antibody.
|
| 反应种属 |
Human, Mouse, Rat
|
| 应用 |
IF~~1:25
FC~~1:25 WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 56kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
889CT6.2.1
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cell Biology
|
| 靶标 | |
| 背景说明 |
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
|
| 细胞定位 |
Cytoplasm. Cell membrane; Peripheral membrane protein
|
| UniProt |
P54578
|
| 参考文献 | |
| 参考文献 |
Deshpande K.L., et al. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. Reuter T.Y., et al. Exp. Cell Res. 289:211-221(2003). Carrascal M., et al. J. Proteome Res. 7:5167-5176(2008). Koulich E., et al. Mol. Biol. Cell 19:1072-1082(2008). |
实验结果图
USP14 Antibody (N-term)(Cat. #JP100375) western blot analysis in K562,Jurkat cell line ,mouse brain abd rat brain tissue lysates (35μg/lane).This demonstrates the USP14 antibody detected the USP14 protein (arrow).