Rabbit anti-MAP3K5(T842)Polyclonal Antibody别名宿主特异性反应种属预测反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 别名 |
MAP3K5抗体;Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; MAP3K5; ASK1; MAPKKK5; MEKK5
|
| 宿主 |
Rabbit
|
| 特异性 |
This MAP3K5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 821-849 amino acids from human MAP3K5.
|
| 反应种属 |
Human
|
| 预测反应种属 |
Mouse
|
| 应用 |
WB~~1:1000
IHC-P~~1:10~50 |
| 分子量 |
Predicted molecular weight: 155kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
|
| 研究领域 |
Cardiovascular;Cell Biology;Apoptosis
|
| 靶标 | |
| 背景说明 |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
|
| 细胞定位 |
Cytoplasm. Endoplasmic reticulum. Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region
|
| UniProt |
Q99683
|
| 参考文献 | |
| 参考文献 |
Min,W.,Circ. Res. 102 (7), 840-848 (2008)
Li,X.,Cell Death Differ. 15 (4), 739-750 (2008) Noguchi,T.,J. Biol. Chem. 283 (12), 7657-7665 (2008) |
实验结果图
All lanes : Anti-MAP3K5 Antibody (T842) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 155 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP3K5 ANtibody (T842) (Cat.#AP7911g), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody (Cat.#AP7911g).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).