LY 294002, 4′-NH2 is a cell permeable 4′-amino derivative which inhibits the p110α, p110β, p110δ, and p110γ subunits of PI 3-kinase. LY 294002, 4′-NH2 functions by preventing the phosphorylation of Akt1 in the PI 3-kinase/Akt1/mTOR pathway. In addition, the 4′ end of LY 294002, 4′-NH2 can be immobilized on an epoxy containing solid surface, which is beneficial for binding studies. Moreover, studies suggest that LY 294002, 4′-NH2 interacts with non-PI 3-kinase cellular proteins such as mTOR, ALDH1, Brd4, GSK3β and CKIIα. Another useful characteristic of LY 294002, 4′-NH2 is that it functions as a reversible inhibitor of PI 3-kinase.

≥98%

Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.

Solid

LY 294002, 4′-NH2

Off-white solid

PI 3-kinase p110β: IC₅₀ = 0.10 µM; PI 3-kinase p110α: IC₅₀ = 0.18 µM; PI 3-kinase p110δ: IC₅₀ = 0.23 µM; PI 3-kinase p110γ: IC₅₀ = 1.97 µM

Soluble in DMSO (100 mM).