NSC-207895 (XI-006) is a derivative of nitrobenzofuroxan and an anticancer agent with an EC50 of 1 μM. NSC-207895 (XI-006) is a highly electrophilic compound known to have both antitumor and mutagenic activities. NSC-207895 (XI-006) may lead to the damage of DNA that inhibits the growth of tumors. NSC-207895 (XI-006) strikingly prevented the expression of MDMX which is a p53-binding protein and modulate the activities and stabilities of p53. NSC-207895 (XI-006) is less toxic and reduced the activities of MDMX promoter in a dose-dependent manner. Moreover,NSC-207895 (XI-006)  possibly decreased the expression of MDMX via suppressing MDMX transcription. However, NSC-207895 (XI-006) induced the expression of MDM2 which is also a p53 downstream gene, despite to a less extent. In addition, NSC-207895 (XI-006) also upregulated the expression of p53 in MCF-7 cells that led to increased expression of proapoptotic genes including PUMA, BAX, as well as PIG3. Notably, NSC-207895 (XI-006), also called a small-molecule p53 activator, gives rise to MCF-7 cells to experience apoptosis and decrease the viability of cancer cells along with nutlin-3a, which dissociated the MDM2-p53 complex.

>98%

Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.

XI-006

DMSO ：0.4 mg/mL (1.43 mM)

p53,MDMX

NSC-207895 decreases both the MDMX mRNA and protein in MCF-7 cells. NSC-207895 induces expression of p53 as well as well-characterized p53-target gene, p21 and MDM2, in a dose-dependent manner in MCF-7 cells. NSC-207895 extends the half-life of p53 from 20 to 30 minutes to more than 3 hours as revealed by cycloheximide chase assays in MCF-7 cells. NSC-207895 also activates p53 and induces p21 and MDM2 expression in LNCaP prostate and A549 lung cancer cells. NSC-207895 increases the mRNA levels of proapoptotic genes including PUMA, BAX, and PIG3 in a dose-dependent manner in MCF-7 cells. NSC-207895 results in a significant increase in the numbers of sub-G0/G1 cells as well as G2 arrest. NSC-207895 also results in more than 40% of cells dying via apoptosis and decreases cell viability in A549 and LNCaP cells. NSC-207895 inhibits biosynthesis of nucleic acids and proteins in L1210 cells. NSC-207895 interacts with DNA repair to activate the DNA damage repair pathway in three species (S. cerevisiae, S. pombe and H. sapiens). NSC-207895 acts as cytotoxic agent in the G/R-luc astrocytoma cell line with GI50 of 117 nM.