Mouse anti-ENO1 Monoclonal Antibody(786CT6.6.4)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
ENO1抗体;Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; ENO1; ENO1L1; MBPB1; MPB1
|
| 宿主 |
Mouse
|
| 特异性 |
Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.
|
| 反应种属 |
Human, Mouse
|
| 应用 |
IHC-P~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 47kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
786CT6.6.4
|
| 同种型 |
IgG2b,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cancer;Metabolism;Signal Transduction
|
| 靶标 | |
| 背景说明 |
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
|
| 细胞定位 |
Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line
|
| UniProt |
P06733
|
| 参考文献 | |
| 参考文献 |
Giallongo A., et al. Proc. Natl. Acad. Sci. U.S.A. 83:6741-6745(1986).
Giallongo A., et al. Eur. J. Biochem. 190:567-573(1990). Ray R., et al. Mol. Cell. Biol. 11:2154-2161(1991). Walter M., et al. J. Autoimmun. 8:931-945(1995). Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. |
实验结果图
Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody(Cat#JP100334). JP100334 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
ENO1 Antibody (Cat. #JP100334) western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35μg/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).