Rabbit anti-Phospho-Rb(Ser780) Monoclonal Antibody(8H7)别名宿主反应种属应用分子量免疫原形式纯化方法克隆号同种型储存/保存方法存储溶液修饰背景说明UniProt
| 概述 | |
| 别名 |
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human, Rat
|
| 应用 |
WB: 1/500-1/1000, IP: 1/20
|
| 分子量 |
Detects a band of approximately 110 kD(Predicted molecular weight: 106 kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb
|
| 性能 | |
| 形式 |
Monoclonal Antibody
|
| 纯化方法 |
Affinity Purified
|
| 克隆号 |
8H7
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
|
| 存储溶液 |
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
|
| 修饰 |
Phosphorylated
|
| 靶标 | |
| 背景说明 |
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
|
| UniProt |
P06400
|
实验结果图
Western blot analysis of Phospho-Rb (Ser780) in K562, C6 lysates using Phospho-Rb (Ser780) antibody.