Rabbit anti-ERBB2 Polyclonal Antibody(Center S728)

Rabbit anti-ERBB2 Polyclonal Antibody(Center S728)别名宿主特异性反应种属预测反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献

概述
别名
ERBB2抗体;Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2; MLN19; NEU; NGL
宿主
Rabbit
特异性
This ERBB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-735 amino acids from the Central region of human ERBB2.
反应种属
Human
预测反应种属
Rat
应用
WB~~1:500
分子量
Predicted molecular weight: 138kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
性能
类型
Polyclonal Antibody
同种型
Rabbit Ig
储存/保存方法
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
存储溶液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
研究领域
Cancer Antibodies;Signal Transduction;Others
靶标
背景说明
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
细胞定位
Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1 Isoform 3: Cytoplasm. Nucleus.
UniProt
P04626
参考文献
参考文献
Geradts, J., et al. Cancer Invest. 28(9):969-977(2010)
Zaoui, K., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18517-18522(2010)
Oliveras, G., et al. Ann. N. Y. Acad. Sci. 1210, 86-92 (2010) :
Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010)
Stackievicz, R., et al. Isr. Med. Assoc. J. 12(5):290-295(2010)

实验结果图

Rabbit anti-ERBB2 Polyclonal Antibody(Center S728)

Anti ERBB2 Antibody (S728) at 1:500 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.