Mouse anti-UCHL1 Monoclonal Antibody(C-term)(696CT2.1.4)描述别名宿主特异性反应种属预测反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
UCHL1 抗体, PGP9.5抗体;Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; 6—; Neuron cytoplasmic protein 95; PGP 95; PGP95; Ubiquitin thioesterase L1; UCHL1
|
| 宿主 |
Mouse
|
| 特异性 |
This UCHL1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.
|
| 反应种属 |
Human, Rat
|
| 预测反应种属 |
B, Pr, M, P
|
| 应用 |
IHC-P~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 25kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
696CT2.1.4
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cell Biology;Neuroscience
|
| 靶标 | |
| 背景说明 |
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
|
| 细胞定位 |
Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain
|
| UniProt |
P09936
|
| 参考文献 | |
| 参考文献 |
Boudreaux D.A., et al. Proc. Natl. Acad. Sci. U.S.A. 107:9117-9122(2010).
Hillier L.W., et al. Nature 434:724-731(2005). Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Day I.N.M., et al. Biochem. J. 268:521-524(1990). Choi J., et al. J. Biol. Chem. 279:13256-13264(2004). |
实验结果图
Immunohistochemical analysis of paraffin-embedded H. brain section using UCHL1 Antibody (C-term)(Cat#JP100354). JP100354 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. astroglioma section using UCHL1 Antibody (C-term)(Cat#JP100354). JP100354 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
UCHL1 Antibody (C-term)(Cat. #JP100354) western blot analysis in 293T,NCI-H1299,rat PC-12 cell line lysates (35μg/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).