Mouse anti-BID Monoclonal Antibody(1519CT649.18.47)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
BID抗体;BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID
|
| 宿主 |
Mouse
|
| 特异性 |
This BID antibody is generated from a mouse immunized with a recombinant protein of human BID.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:4000
IF~~1:25 IHC-P~~1:25 |
| 分子量 |
Predicted molecular weight: 22kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1519CT649.18.47
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Cancer;Cell Biology;Metabolism
|
| 靶标 | |
| 背景说明 |
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
|
| 细胞定位 |
Cytoplasm. Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. BH3-interacting domain death agonist p13: Mitochondrion membrane. Note=Associated with the mitochondrial membrane. Isoform 3: Cytoplasm.
|
| UniProt |
P55957
|
| 参考文献 | |
| 参考文献 |
Wang K.,et al.Genes Dev. 10:2859-2869(1996).
Footz T.K.,et al.Genomics 51:472-475(1998). Renshaw S.A.,et al.J. Biol. Chem. 279:2846-2855(2004). Dai F.Y.,et al.Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004). |
实验结果图
All lanes : Anti-BID Antibody at 1:4000 dilution Lane 1: Jurkat whole cell lysates Lane 2: A431 whole cell lysates Lane 3: THP-1 whole cell lysates Lane 4: 293T/17 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.