Mouse anti-CD28 Recombinant Monoclonal Antibody(AF647 Conjugate)(15E8)别名宿主反应种属应用形式浓度纯化方法类型克隆号同种型储存/保存方法存储溶液标记背景说明细胞定位UniProt
| 概述 | |
| 别名 |
TP44 (Alexa Fluor® 647 Conjugate)
|
| 宿主 |
Mouse
|
| 反应种属 |
Human
|
| 应用 |
FC (Extra): 1:2500
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
2.5 mg/mL
|
| 纯化方法 |
Protein G affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1.50E+09
|
| 同种型 |
IgG1,k
|
| 储存/保存方法 |
Store at 2-8℃ for one year.
|
| 存储溶液 |
PBS, 0.1% BSA, 0.01% Proclin 300
|
| 标记 |
Alexa Fluor® 647
|
| 靶标 | |
| 背景说明 |
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen-presenting cells (APCs). The CD86 expression on antigen-presenting cells is constitutive (expression is independent of environmental factors). It is generally reported, that CD28 is expressed on 50% of CD8+ T cells and more than 80% CD4+ T cells in human, but during the course of activation some T cells lose this molecule. In general, CD28 is a primary costimulatory molecule for T cell activation.
|
| 细胞定位 |
Membrane
|
| UniProt |
P10747
|
实验结果图
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte, left)/ Jurkat (Human T cell leukemia T lymphocyte, right) cells labelling CD28 (Alexa Fluor 647 Conjugate) antibody at 1/2500 (0.1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: THP-1