Rabbit anti-SAE2 Polyclonal Antibody(C-term E616)描述别名宿主特异性反应种属应用分子量类型同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Rabbit Polyclonal Antibody (Pab)
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| 别名 |
SAE2抗体;SUMO-activating enzyme subunit 2; 632-; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; UBA2; SAE2; UBLE1B
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| 宿主 |
Rabbit
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| 特异性 |
This SAE2 (UBA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 601-631 amino acids from the C-terminal region of human SAE2 (UBA2).
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| 反应种属 |
Human
|
| 应用 |
WB~~1:1000
IHC-P~~1:50~100 |
| 分子量 |
Predicted molecular weight: 71kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Polyclonal Antibody
|
| 同种型 |
Rabbit Ig
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| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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| 存储溶液 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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| 研究领域 |
Cell Biology
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| 靶标 | |
| 背景说明 |
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
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| 细胞定位 |
Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention
|
| UniProt |
Q9UBT2
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| 参考文献 | |
| 参考文献 |
Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999). Gong, L., et al., FEBS Lett. 448(1):185-189 (1999). Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
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实验结果图
The anti-UBA2 C-term E616 Antibody (Cat.#JP100755) is used in Western blot to detect UBA2 in Jurkat lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.