Mouse anti-RAB5C Monoclonal Antibody(1616CT314.65.31)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
概述 | |
描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
别名 |
RAB5C抗体;Ras-related protein Rab-5C; L1880; RAB5L; RAB5C; RABL
|
宿主 |
Mouse
|
特异性 |
This RAB5C antibody is generated from a mouse immunized with a recombinant protein of human RAB5C.
|
反应种属 |
Human
|
应用 |
FC~~1:25
WB~~1:2000 |
分子量 |
Predicted molecular weight: 23kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
性能 | |
类型 |
Monoclonal Antibody
|
克隆号 |
1616CT314.65.31
|
同种型 |
IgG2b,k
|
储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
研究领域 |
Signal Transduction
|
靶标 | |
背景说明 |
Protein transport. Probably involved in vesicular traffic (By similarity).
|
细胞定位 |
Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
|
UniProt |
P51148
|
参考文献 | |
参考文献 |
Han H.J.,et al.Cytogenet. Cell Genet. 73:137-139(1996).
Albertsen H.M.,et al.Nat. Genet. 7:472-479(1994). Clemens D.L.,et al.Infect. Immun. 68:2671-2684(2000). Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). |
实验结果图
All lanes : Anti-RAB5C Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.