Rabbit anti-sTfR Recombinant Monoclonal Antibody(S-612-12)描述别名宿主特异性反应种属预测反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明组织特异性翻译后修饰细胞定位UniProt
| 概述 | |
| 描述 |
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection.
|
| 别名 |
CD71抗体, TfR抗体;T9; p90; CD71
|
| 宿主 |
Rabbit
|
| 特异性 |
CD71/TfR Antibody detects endogenous levels of total CD71/TfR.
|
| 反应种属 |
Human
|
| 预测反应种属 |
Pig;Rabbit;Sheep;Dog;Bovine;Horse;
|
| 应用 |
WB: 1:1000, IHC-P: 1:2000
|
| 免疫原 |
Recombinant Protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
S-612-12
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Soluble transferrin receptor conventionally refers to the cleaved extracellular portion of transferrin receptor 1 that is released into serum. This receptor is a protein dimer of two identical subunits, linked together by two pairs of disulfide bonds. Its molecular mass 190,000 Dalton. Blood testing of the soluble transferrin receptor (sTfR) is used as a measure of functional iron status and the investigation of iron deficiency anemia. Ferritin, a routine investigation for anemia, is an acute-phase reactant, and may be elevated in states of inflammation, thereby falsely indicating that iron stores are adequate. Because sTfR is insensitive to inflammation, it can detect anemia in patients with preexisting inflammatory states, and is particularly useful in distinguishing between the anemia of chronic disease and anemias caused by lack of iron intake.
|
| 组织特异性 |
Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3′-UTR. Up-regulated upon mitogenic stimulation.
|
| 翻译后修饰 |
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
|
| 细胞定位 |
Cell membrane
|
| UniProt |
P02786
|
实验结果图
WB result of sTfR Rabbit mAb Primary antibody: sTfR Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: K562 whole cell lysate 20 ug Lane 3: HT-1080 whole cell lysate 20 ug Lane 4: 293T whole cell lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 84 kDa Observed MW: 100, 85 kDa Exposure time: 180 s
IHC shows positive staining in paraffin-embedded human kidney. Anti-sTfR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-sTfR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-sTfR antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.