Mouse anti-CYK18 Monoclonal Antibody(C-term) (608CT2.1.3)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
CYK18抗体;Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18; KRT18; CYK18
|
| 宿主 |
Mouse
|
| 特异性 |
This CYK18 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 400-430 region of human CYK18.
|
| 反应种属 |
Human
|
| 应用 |
IHC-P~~1:25
IF~~1:25 WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 48kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
695CT17.2.6
|
| 同种型 |
IgG3
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Signal Transduction
|
| 靶标 | |
| 背景说明 |
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
|
| 细胞定位 |
Cytoplasm, perinuclear region. Nucleus, nucleolus
|
| UniProt |
P05783
|
| 参考文献 | |
| 参考文献 |
Oshima R.G., et al. Differentiation 33:61-68(1986).
Kim J.W., et al. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases. Kalnine N., et al. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Suzuki Y., et al. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Kulesh D.A., et al. Mol. Cell. Biol. 8:1540-1550(1988). |
实验结果图
All lanes : Anti-CYK18 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCI-H460 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-CYK18 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NCI-H460 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.