Mouse anti-ADA Monoclonal Antibody(C-term)(608CT2.1.3)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
ADA抗体;Adenosine deaminase; Adenosine aminohydrolase; ADA; ADA1
|
| 宿主 |
Mouse
|
| 特异性 |
This ADA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA.
|
| 反应种属 |
Human
|
| 应用 |
WB~~1:100~250
|
| 分子量 |
Predicted molecular weight: 41kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
608CT2.1.3
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Cancer;Metabolism
|
| 靶标 | |
| 背景说明 |
Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion.
|
| 细胞定位 |
Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Note=Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion
|
| UniProt |
P00813
|
| 参考文献 | |
| 参考文献 |
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Gloria-Bottini, F., et al. Am. J. Med. Sci. 340(2):103-108(2010) Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010) Spina, C., et al. Cancer Invest. (2010) In press : Ri, G., et al. Anticancer Res. 30(6):2347-2349(2010) |
实验结果图
All lanes : Anti-ADA Antibody (C-term) at 1:4000 dilution Lane 1: Jurkat whole cell lysate Lane 2: CEM whole cell lysate Lane 3: MOLT-4 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
ADA Antibody (C-term)(Cat. #JP100288) western blot analysis in CEM,Jurkat cell line lysates (35μg/lane).This demonstrates the ADA antibody detected the ADA protein (arrow).