Rabbit anti-GLUT-1 Recombinant Monoclonal Antibody(AF594 Conjugate)(047-53)别名宿主反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液标记背景说明细胞定位UniProt
| 概述 | |
| 别名 |
SLC2A1 (Alexa Fluor® 594 Conjugate); GLUT1 (Alexa Fluor® 594 Conjugate)
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
ICC: 1:500, FC (Intra): 1:200
|
| 免疫原 |
Synthetic Peptide
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
2 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
047-53
|
| 储存/保存方法 |
Store at 2-8℃ for one year.
|
| 存储溶液 |
PBS, 0.1% BSA, 0.01% Proclin 300
|
| 标记 |
Alexa Fluor® 594
|
| 靶标 | |
| 背景说明 |
Glucose enters cells through various transporters including the GLUT family of facilitative transporters, the sodium/glucose co-transporters, and the recently discovered SWEET family. however, only the role of the GLUT family has been extensively studied in cancer cells. There are 14 GLUT proteins in the human and 12 in the mouse. GLUT1, encoded by SLC2A1, is the predominant transporter overexpressed in tumors, including hepatic, pancreatic, esophageal, brain, renal, lung, cutaneous, colorectal, endometrial, ovarian, cervical, and breast, as well as head and neck tumors.
|
| 细胞定位 |
Melanosome, Cell Membrane
|
| UniProt |
P11166
|
实验结果图
ICC shows positive staining in HepG2 cells. Anti-GLUT1 (Alexa Fluor® 594 Conjugate) antibody was used at 1/500 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 cells labelling GLUT-1 (Alexa Fluor 594 Conjugate) antibody at 1/200 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).