Rabbit anti-GCDFP-15 Monoclonal Antibody(010-579)别名宿主反应种属应用分子量免疫原形式浓度纯化方法克隆号同种型储存/保存方法存储溶液研究领域背景说明UniProt
| 概述 | |
| 别名 |
巨囊性病液体蛋白-15;Prolactin-inducible protein; Secretory actin-binding protein (SABP); gp17; GPIP4; PIP
|
| 宿主 |
Rabbit
|
| 反应种属 |
Human
|
| 应用 |
WB: 1:1000
IHC-P: 1:250 |
| 分子量 |
16kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 |
Synthetic peptide
|
| 性能 | |
| 形式 |
liquid
|
| 浓度 |
0.5mg/ml
|
| 纯化方法 |
Protein A affinity column
|
| 克隆号 |
010-579
|
| 同种型 |
IgG
|
| 储存/保存方法 |
Store at 2-8℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
|
| 研究领域 |
Immunology
|
| 靶标 | |
| 背景说明 |
Gross cystic disease fluid protein 15 (GCDFP-15) also known as Prolactin-inducible protein, extra-parotid glycoprotein (EP-GP), gp17 seminal actin-binding protein (SABP) or BRST2 is a protein that in humans is encoded by the PIP gene. It is upregulated by prolactin and androgens and downregulated by estrogen. The protein has a physiological function in regulation of water transport mainly in apocrine glands in the axilla, vulva, eyelid and ear canal, serous cells of the submandibular salivary gland, serous cells of the submucosal glands of the bronchi, and accessory lacrimal glands as well as cutaneous eccrine glands. It is also found in amniotic fluid and seminal fluid. PIP has the ability to bind immunoglobulin G (IgG), IgG-Fc, CD4-T cell receptor suggesting a wide range of immunological functions. PIP also binds to AZGP1. PIP exerts aspartyl proteinase activity able to cleave fibronectin. Mitogenic effect of PIP was observed on both normal and malignant breast epithelial cells.
|
| UniProt |
P12273
|
实验结果图
Primary antibody : Anti-GCDFP-15 antibody at 1/1000 dilution Lane 1 : MCF-7 whole cell lysate 20 µg Lane 2 : T47D whole cell lysate 20 µg Negative control: MCF-7 whole cell lysate Secondary antibody: #JP20040 at 1/10000 dilution Predicted MW: 16 kDa Observed MW: 16 kDa Exposure time: 9 seconds”
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GCDFP-15 antibody was used at 1/250 dilution, Secondary antibody: #JP20040 Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human colon (negative tissue). Anti-GCDFP-15 antibody was used at 1/250 dilution, Secondary antibody: #JP20040. Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.