GW5074CAS号: 220904-83-6分子式: C15H8Br2INO2分子量: 520.94描述纯度储存/保存方法别名外观可溶性/溶解性靶点In vitro(体外研究)In vivo(体内研究)
产品描述 | |
描述 |
GW 5074是可渗透入细胞的c-Raf1激酶选择性抑制剂,IC50为9nM,有超过100倍的选择性对CDK1,CDK2,c-src,ERK2,MEK,p38,Tie2,VEGFR2和c-fms。 |
纯度 |
98%
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储存/保存方法 |
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
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基本信息 | |
别名 |
GW-5074; GW 5074
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外观 |
yellow to orange-brown solid
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可溶性/溶解性 |
DMSO : 96 mg/mL (184.3 mM)
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生物活性 | |
靶点 |
C-Raf
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In vitro(体外研究) |
GW5074 is a potent and specific inhibitor of c-Raf with IC50 of 9 nM and has no effect of MKK6, MKK7, p38 MAP kinase and cdks in vitro. However, treatment of neuronal cultures with GW5074 permits accumulation of activating modifications on c-Raf and also B-Raf. The inhibition of LK-induced apoptosis by GW5074 in cerebellar granule neurons is not MEK-ERK-dependent. GW5074 delays down-regulation of Akt activity but inhibits apoptosis by an Akt-independent mechanism. GW5074 affects Ras, nuclear factor-kappa B and c-jun. GW5074 inhibits cell death caused by neurotoxins in granule cells and other neuronal types.
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In vivo(体内研究) |
GW5074 is protective in an in vivo experimental model of Huntington’s disease. GW5074 (5 mg/Kg) completely prevented extensive bilateral striatal lesions induced by 3-NP in mice. GW5074 completely abolishes chronic morphine-mediated AC superactivation I in CHO cells stably expressing the humanμ-opioid receptor. GW5074 suppresses sidestream smoke-induced airway hyperresponsiveness in mice.
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分子结构图