NCT-504, a selective allosteric inhibitor of PIP4Kγ, exhibits an IC50 value of 15.8 μM. It holds promise for the investigation of Huntington’s disease.

98%

Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.

DMSO:112.5mg/mL (278.1mM)

PIP4Kγ:15.8 μM (IC50)

NCT-504 dose not impair the intrinsic ATP-hydrolytic activity of PIP4Kγ in the JPence of PI5P substrate [1] . NCT-504 does not inhibit PIP4Kbeta and weakly inhibits PIP4Kalpha phosphorylation of PI5P [1] . NCT-504 dose not inhibit PIP4Kbeta or PIP4Kalpha (IC 50 between 50 μM and 100 μM) at 50 μM concentration [1] . NCT-504 elevates the levels of PI(3,5)P2, PI3P and PI5P in MEFs [1] . NCT-504 (10 μM; 12 hours) does not affect cell viability in MEFs [1] . NCT-504 (5 μM, 10 μM; 2 hours, 6 hours) elevates both the induction of autophagy as well as the rate of turnover of autophagic cargo [1] . NCT-504 treatment causes a robust increase in the formation of autolysosomes with only a modest elevation in autophagosomes [1] . NCT-504 increases autophagy flux and decreases huntingtin protein in 293A cells [1] . NCT-504 reduces mHtt protein levels in immortalized striatal cells from knock-in HD mice [1] . Western Blot Analysis [1] Cell Line: HEK293T cells Concentration: 5 μM, 10 μM Incubation Time: 2 hours, 6 hours Result: Induced autophagosome formation.