细胞培养基 DMEM(H)(含双抗)12100-500

细胞培养基 DMEM(H)(含双抗)12100-500

产品名称:细胞培养基 DMEM(H)(含双抗)

产品型号:12100-500

产品特点:细胞培养基 DMEM(H)(含双抗)
产品含4.5g/L 葡萄糖 ,含谷氨酰胺,不含丙酮酸钠。质量稳定可靠

12100-500细胞培养基 DMEM(H)(含双抗)的详细资料:

细胞培养基 DMEM(H)(含双抗)

 

DMEM培养基的配制方法:

1 制备新鲜三蒸水或Millipore超纯水。

2 称取所需量的干粉培养基,加入约终体积一半的三蒸水中;若配制一个包装的培养液,在将整个包装的干粉倒入三蒸水后,需用水洗包装袋内面2次,倒入培养液中,以保证所有干粉都溶解成培养液。磁力搅拌或人工搅拌使之*溶解。

3 根据包装袋上的要求补加所需量的碳酸氢钠;根据实验需要,添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物质。

4 加水定容到终体积。

5 必要时用1 mol/L 盐酸和1 mol/L 氢氧化钠调节pH。

6 用无菌0.22um滤膜过滤除菌,分装于无菌血清瓶中,4℃冰箱保存。

配制好的培养液用前加入100U/mL青霉素和100U/mL链霉素,并根据需要加入血清(5%-20%)。

DMEM培养基的特点:

(1)氨基酸含量为依格尔培养基的2倍,且含有非必需氨基酸,如甘氨酸等;

(2)维生素含量为依格尔培养基的4倍;

(3)含有糖酵解途径中的重要物质——丙酮酸;

(4)含有微量的铁离子。

DMEM培养基应用广泛。基于DMEM培养基中氨基酸、微生物含量丰富,并具有糖酵解中的丙酮酸和微量Fe离子,因此在细胞培养、疫苗生产等方面的应用为常见。

适用于细胞培养, 产品含4.5g/L 葡萄糖 ,含谷氨酰胺,不含丙酮酸钠。质量稳定可靠。

DMEM (Dulbecco's Modified Eagle Medium) (high Glucose)

with 4.5g/L Glucose,with  L- glutamine,without sodium pyruvate

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细胞培养基 DMEM(H)(含双抗)