Shibayagi 小鼠 C-肽 ELISA试剂盒(U型)

Shibayagi 小鼠 C-肽 ELISA试剂盒(U型)
Lbis® C-Peptide-Mouse (U type)

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Lbis® C-Peptide-Mouse (U type)Shibayagi 小鼠 C-肽 ELISA试剂盒(U型)

Shibayagi 小鼠 C-肽 ELISA试剂盒(U型)

  胰岛素是细胞中的单链胰岛素原合成后,形成二硫键,通过酶分解激活,裂解成C肽与胰岛素。小鼠、大鼠的胰岛素的氨基酸序列相同,但C肽部分稍有不同。小鼠C肽1是29个氨基酸,2是31个的单链肽。C肽是从胰岛素原分离后,与胰岛素一同分泌生成的。长期以来,人们一直认为C肽没有生物活性,仅在合成胰岛素过程中,保证A链和B链正确折叠以及二硫键正确配对时起作用。近年,随着研究的不断深入,证明了C肽具有多种生物学作用。首先,10-9M的C肽能与内皮细胞、肾小管上皮细胞和成纤维细胞表面的G蛋白偶联受体结合。激活细胞中钙离子依赖性的信号、激活Na-K-ATPase、促进内皮细胞的NO合成、与受体的结合有立体结构特异性;与胰岛素、胰岛素原、IGF-I、-II、NPY之间无交叉反应。而且通过对缺少C肽的Ⅰ型糖尿病患者注射C肽类药物,能起到增强骨骼肌以及皮肤的血液循环、降低肾小球超滤的风险、抑制白蛋白从尿液中的排泄、改善神经机能的作用。但对身体健康的人来说,这类药物没有此等功效。因此,建议Ⅰ型糖尿病患者可以在注射胰岛素的同时,投放C肽,有利于防止并发症的发生。

  C肽的C末端的五肽(27-31)在与受体的结合中起着重要的作用,缺少这部分的Des(27-31)C肽就会失去它的作用。这种五肽可以完全取代C肽和受体的结合,激活Na+-K+ATPase。有报告指出新生大鼠中Des(27-31)C肽的存在量约占C肽总量的37%,而在成年大鼠中只占8.5%。

  C肽在血液中的寿命是胰岛素的好几倍。在临床上,可以通过测量C肽在血液中的浓度来观察胰岛素的合成和分泌功能。且C肽在尿液中多量排除,一定程度上与血液中C肽的平均值相关,所以也可以通过尿液来检测。

  作为人工胰岛中胰岛素分泌的指标,C肽测量是有效的。由于在培养液中经常添加胰岛素,如果要测量培养后培养液中的胰岛素的话,就不能很好地区别出分泌的胰岛素和添加的胰岛素,必须减掉培养开始时胰岛素的量。这种情况下,如果分泌的胰岛素量过少,测量误差的影响会增大,从而不能做出正确的判断。这时候,如果测量C肽,因为C肽与胰岛素是等摩尔分泌的,所以能够正确判断分泌的胰岛素。

  本公司的整套产品能够识别C肽1、2的交叉部分,可以测量C肽的总量。

◆特点

● 短时间测定(完全反应时间:5小时)

● (标准用量10 μL)可测

● 使用对环境无害的防腐剂

● 全部试剂均为液体,可直接使用

● 精密的测定密度和高再现性

◆构成

组成

状态

容量

(A) 抗体固相化   96 孔板

洗净后使用

96 wells(8×12)/1 块

(B) 标准溶液(6,000 pg/mL)

稀释后使用

500 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 抗C-肽抗体生物素结合

稀释后使用

100 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL/1 瓶

(F) 显色液(TMB)

即用

12 mL/1 瓶

(H) 反应停止液(1M   H2SO4)※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液(10×)

稀释后使用

100 mL/1 瓶

封板膜

4 张

使用说明书

1 份

◆样本

小鼠的血清或血浆

10 μL/well(用本品配备的缓冲液稀释后、50 μL分注在孔板中。)

◆测定范围

46.9~3,000 pg/mL(标准曲线范围)

234.5~15,000 pg/mL(检体量10 μl的时候)

◆Validation data

 

精度测试(检测内变异系数)

 

样本

A

B

1

976

238

2

969

230

3

965

230

4

1023

235

5

977

231

6

1018

228

7

1038

229

8

995

225

mean

995

231

SD

27.7

4.10

CV(%)

2.78

1.78

单位:pg/mL

再现性测试(检测内变异系数)

 

测量日/检体

C

D

E

第0天

1502

301

60.9

第1天

1500

302

63.8

第2天

1499

301

62.2

第3天

1501

300

58.8

mean

1500

301

61.4

SD

1.12

0.66

2.13

CV(%)

0.07

0.22

3.46

单位:pg/mL, n=4

添加回收测试

 

样本F

添加量

实测值

回收量

回收率(%)

0.00

300

265

551

250

94

398

683

382

96

531

827

527

99

单位:pg/mL, n=2

样本G

添加量

实测值

回收量

回收率(%)

0.00

58.2

28.9

86.7

28.5

99

38.6

98.2

40.0

104

77.4

139

80.8

104

单位:pg/mL, n=2

稀释直线性测试

用稀释缓冲液分三次连续稀释2个血清检体的结果,直线回归方程的R2是1.00。

欲了解更多相关产品信息,请点击文字:Lbis® 疾病相关动物模型ELISA试剂盒系列

相关资料


Shibayagi 小鼠 C-肽 ELISA试剂盒(U型) Shibayagi 小鼠 C-肽 ELISA试剂盒(U型) Shibayagi 小鼠 C-肽 ELISA试剂盒(U型)
说明书

ELISA试剂盒选择指南①②

ELISA试剂盒选择指③④

参考文献


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产品编号 产品名称 产品规格 产品等级 产品价格
635-07239 (AKRCP-031)Lbis® Mouse C-peptide ELISA kit (U-type)
Lbis®小鼠 C-肽 ELISA试剂盒(U型) 
96 tests