Cy5-N-羟基琥珀酰亚胺酯|Cyanine5 NHS ester|金畔生物


Cy5-N-羟基琥珀酰亚胺酯

货号:AGF1338A-5mg
包装:5mg

  • 英文名:Cyanine5 NHS ester
  • 级别:NMR 1H (95%) and 13C, TLC, functional testing
  • 分子式:C36H42ClN3O4
  • 分子量:616.19
  • 品牌:lumiprobe
  • CAS:1032678-42-4, 350686-88-3
  • 外观:dark blue powder
  • 产品详情质检信息

    订购货号 产品名称及规格
    13020 Cyanine5 NHS ester, 1 mg
    23020 Cyanine5 NHS ester, 5 mg
    43020 Cyanine5 NHS ester, 25 mg
    53020 Cyanine5 NHS ester, 50 mg
    63020 Cyanine5 NHS ester, 100 mg

    在过去的几年里,CY5荧光团已经成为生命科学研究和诊断领域非常流行的分子标记物。这些荧光素的最强发射光为红光,在这个范围大多数CCD检测器具有最大检测灵敏度,而且是生物物质低背景区。染料的颜色是非常强烈的,因此在凝胶电泳中低至1nmol的量都能被肉眼观测到。CY5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。在标记反应中该染料需要少量的有机共溶剂(如DMF,DMSO)(请参照我们的推荐手册以获得更多帮助信息)。对于可溶性蛋白、各种各样的多肽和寡核苷酸而言,这种染料是非常理想而且成本低廉的。该染料在有机溶剂对小分子物质的标记也是非常有效的。对于更多的精细目标物,如易降解蛋白,如果DMF或DMSO会对其有影响,可以考虑water-soluble Cy5 NHS ester(水溶性Cy5 NHS ester染料),因为它们不需要有机溶剂助溶,而且具有和荧光染料类似的性质。

    CY5荧光团可以与很多的仪器如荧光显微镜、成像仪、扫描仪、荧光分析仪等相兼容。许多的CY5衍生物——CY5 NHS ester可以替代Alexa Fluor 647和 DyLight 649等活化酯(activated esters)。 

    Cy5-N-羟基琥珀酰亚胺酯|Cyanine5 NHS ester|金畔生物

    推荐手册
    氨基分子的NHS酯标记(请联系金畔生物获取)
    Cy® is a trademark of GE Healthcare.
     

    S" style="color: rgb(61, 61, 61);">General properties

    Appearance: dark blue powder
    Molecular weight: 616.19
    Molecular formula:

    C36H42ClN3O4

    CAS number: 1032678-42-4, 350686-88-3
    Solubility: good in polar (DMSO, DMF) and chlorinated (DCM, chlroform) organic solvents, low solubility in water
    Quality control: NMR 1H (95%) and 13C, TLC, functional testing
    Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
    MSDS: Download
    Spectral properties
    Excitation maximum, nm: 646
    Extinction coefficient at excitation maximum, Lmol-1cm-1: 250000
    Emission maximum, nm: 662
    Fluorescence quantum yield: 0.2

     


    Product citations

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