伯乐TGX Stain-Free™ FastCast™ Acrylamide Solutions | Bio-Rad Laboratories

TGX Stain-Free FastCast acrylamide kits for hand casting polyacrylamide gels enable fast casting, long shelf life gels, short run times, high transfer efficiency, and protein visualization in 5 minutes with stain-free technology.

描述

What Is Stain-Free Technology?

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TGX Stain-Free FastCast premixed acrylamide solutions bring all the benefits of TGX gel chemistry to handcast polyacrylamide gels. TGX (Tris-Glycine eXtended) formulation retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system. TGX Stain-Free FastCast premixed acrylamide solutions for hand casting are available in the most commonly used cross-linking ratios for PAGE systems.

TGX Stain-Free chemistry enables rapid fluorescent detection of proteins directly within gels and blots with Bio-Rad’s stain-free enabled imagers. Protein samples can be separated in 20 minutes and then visualized in 5 minutes.

Features and Benefits of TGX Stain-Free FastCast Acrylamide Solutions

Handcast gels ready to run in as little as 30 min
Long shelf life — handcast gels remain fresh for up to 4 weeks at 4°C
Stain-free visualization of proteins in your gel or blot in less than 5 min with stain-free enabled imaging systems (Gel Doc™ EZ, Gel Doc XR+, ChemiDoc™ MP, ChemiDoc XRS+, and ChemiDoc Touch Systems)
Electrophoresis run times as short as 20 min using the Mini-PROTEAN^® Tetra or Criterion™ cells
Efficient protein transfers in as little as 3 min using the Trans-Blot^® Turbo™ transfer system
Ability to monitor the success of your electrophoresis and transfer steps without additional staining

Applications and Uses of TGX Stain-Free FastCast Acrylamide Solutions

1-D polyacrylamide gel electrophoresis for separation of proteins
Accurate molecular weight estimation
Robust separation for complex samples

Approximate number of gels per kit.

	Mini Gels (~9 x 7 cm)				Midi Gels (~13 x 9 cm)
	Mini-PROTEAN Cassettes	0.75 mm Glass Plates	1.0 mm Glass Plates	1.5 mm Glass Plates	Criteron Cassettes
Starter Kit	11 gels	19 gels	13 gels	9 gels	7 gels
Full Kit	55 gels	95 gels	65 gels	45 gels	35 gels

More Information

Watch the TGX FastCast tutorial video to see how quick and easy it is to cast gels with TGX FastCast acrylamide kits. View step-by-step instructions for casting mini gels using the Mini-PROTEAN Tetra casting module.

TGX Stain-Free™ FastCast™ Acrylamide Solutions | Bio-Rad Laboratories Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.

TGX Stain-Free™ FastCast™ Acrylamide Solutions | Bio-Rad Laboratories Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Stain-Free Imaging

Bio-Rad’s V3 Western Workflow™ is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins. View the V3 Western Workflow video to learn more.

The Quick Start Guide to Stain-Free Imaging provides step-by-step instructions on how to obtain stain-free gel images from TGX Stain-Free gels in less than 5 minutes using Bio-Rad’s ImageLab™ software and stain-free enabled imagers.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Download the FastCast kits calculator to automatically calculate the volumes of stacker, resolver, APS, and TEMED required when casting TGX FastCast and TGX Stain-Free™ FastCast gels.

Learn how to cast better gels with Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits: Tips & Tricks guide.

Bio-Rad Gel Electrophoresis Systems

Gel handcasting equipment and electrophoresis cells are offered in mini and midi gel formats. See the electrophoresis system selection guide for more details.

Midi gel systems:

Criterion and Criterion™ Dodeca™ cells use integral cassettes for gel hand casting. 1.0 mm thick gel casting sets are available in various comb configurations:

12+2 well comb (345-9901)
18-well comb (345-9902)
26-well comb (345-9903)

Related Technologies

Introduction to Protein Electrophoresis
Introduction to Western Blotting

Related Products

TGX Stain-Free™ FastCast™ Acrylamide Solutions
Trans-Blot® Turbo™ 全能型蛋白转印系统

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(停产) TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 7.5%

1610180

Starter kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

(停产) TGX Stain-Free™ FastCast™ Acrylamide Kit, 7.5%

1610181

Kit for hand casting 7.5% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

(停产) TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10%

1610182

Starter kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

(停产) TGX Stain-Free™ FastCast™ Acrylamide Kit, 10%

1610183

Kit for hand casting 10% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

(停产) TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 12%

1610184

Starter kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

(停产) TGX Stain-Free™ FastCast™ Acrylamide Kit, 12%

1610185

Kit for hand casting 12% TGX Stain-Free polyacrylamide gels, includes acrylamide solutions and buffers

配套设备

(停产) Mini-PROTEAN® Vertical Electrophoresis Cell, 4-gel, for 0.75 mm thick handcast gels

1658000FC

4-gel vertical electrophoresis system, 0.75 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

试剂&试剂盒

(停产) Ammonium Persulfate (APS)

1610700

10 g, ammonium persulfate (APS) catalyst

(停产) 2x Laemmli Sample Buffer

1610737

30 ml, premixed protein sample buffer for SDS-PAGE

(停产) 4x Laemmli Sample Buffer

1610747

10 ml, premixed 4x Laemmli protein sample buffer for SDS-PAGE

(停产) 10x Tris/Glycine/SDS

1610772

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3 following dilution to 1x with water

(停产) TEMED

1610800

5 ml, for catalyst for polyacrylamide gels

(停产) TEMED

1610801

50 ml, catalyst for polyacrylamide gels; hazardous material shipping charges may apply

Standards

(停产) Precision Plus Protein™ All Blue Prestained Protein Standards, value pack

1610393

2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications

(停产) Precision Plus Protein™ Dual Color Standards, 2.5 ml

1610394

2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications

(停产) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack

1610395

2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications

(停产) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack

1610397

2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications

(停产) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged, recombinant, 5 ml

1610396

5 ml (5 x 1 ml), unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 500 applications

Plates

(停产) Mini-PROTEAN^® Short Plates

1653308

Pkg of 5, short plate for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(停产) Mini-PROTEAN^® Spacer Plates with 0.75 mm Integrated Spacers

1653310

Pkg of 5, spacer plate with 0.75 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(停产) Mini-PROTEAN^® Spacer Plates with 1.0 mm Integrated Spacers

1653311

Pkg of 5, spacer plate with 1.0 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(停产) Mini-PROTEAN^® Spacer Plates with 1.5 mm Integrated Spacers

1653312

Pkg of 5, spacer plate with 1.5 mm integrated spacer, for Mini-PROTEAN Tetra System and Mini-PROTEAN 3 Multi-Casting Chamber

(停产) Mini-PROTEAN^® Empty Cassettes

4560001

Pkg of 50, cassettes with IPG well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0011)

(停产) Mini-PROTEAN^® Empty Cassettes

4560006

Pkg of 50, cassettes with 15 well marks for casting gels 8.6 x 7.2 cm by 1.0 mm thick, for use with Mini-PROTEAN Electrophoresis Cells; purchase combs separately (456-0015)

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伯乐TGX Stain-Free™ FastCast™ Acrylamide Solutions | Bio-Rad Laboratories

描述

What Is Stain-Free Technology?

Features and Benefits of TGX Stain-Free FastCast Acrylamide Solutions

Applications and Uses of TGX Stain-Free FastCast Acrylamide Solutions

More Information

Stain-Free Imaging

Bio-Rad Gel Electrophoresis Systems

Midi gel systems:

Bio-Rad Gel Electrophoresis Systems

Midi gel systems:

Related Technologies

Related Products

订购

配套设备

试剂&试剂盒

Standards

Plates

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