伯乐Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

Gene Pulser MXcell 电穿孔系统可在多孔板或比色皿上将分子高效传递到哺乳动物细胞中(特别是传递到原生和难于转染的细胞中)。

描述

Gene Pulser MXcell 电穿孔系统既可以采用电击板又可以采用电击杯进行实验,既可采用仪器预设程序,也可由用户
自定义的方式,适用于新的或现有的转化条件,其增强的程序功能可以快速得到理想的转化结果。

主要优点和功能

  • 选择电击板或电击杯转化 — 转化数量较少的原代细胞或进行高通量筛选
  • 转化各种哺乳动物细胞及原代细胞 — 完全开放系统,可以转化 siRNA,DNA 和其他生物分子
  • 预设程序 — 用于快速优化转染条件,增加转化效率和提高细胞存活率,包括对于原代细胞和难转化细胞系的优化
  • 手动编写程序 — 根据细胞类型,如不同原代细胞及干细胞设计程序
  • 兼容 Gene Pulser® 电激缓冲液 — 将 siRNA,DNA 和生物分子高效率导入原代细胞和难转染细胞系

Related Technologies

  • Isolation and Maintenance of Stem Cells

  • Stem Cell Research

  • Transfection and Transduction of Stem Cells

  • Transfection and Transduction of Stem Cells

  • Analysis of Stem Cells

  • Types of Automated Cell Counters

  • Instrument-Based Transfection Methods

  • Cell Counting Methods

Related Products

  • Gene Pulser 电穿孔缓冲液

技术指标

Gene Pulser Xcell™ 完全系统包括主单元、CE 模块、PC 模块和 ShockPod™ 电击槽
 

波型
指数波或方波

电压,V
10–3,000

电容,µF
10–500 V: 25–3,275 µF,增量为 25 µF;500–3,000 V: 10、25、50 µF

Resistance (parallel), Ω
50–1,000 Ω in 50 Ω increments, plus infinity

Sample resistance, Ω
20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V

方波计时
10–500 V: 0.05–10 毫秒,增量为 0.05 毫秒,10–100 毫秒脉冲,增量为 1 毫秒,1–10 次脉冲,0.1–10 秒间隔;500–3,000 V: 0.05–5 毫秒,增量为 0.05 毫秒,1–2 次脉冲,5 秒最小间隔

尺寸(宽 x 深 x 高),cm
主单元: 31 x 30 x 14
CE 模块: 31 x 28 x 9
PC 模块: 31 x 28 x 5

重量,kg
主单元: 6.6
CE 模块: 3.1
PC 模块: 1.9

Gene Pulser Xcell 真核系统 包括主单元、CE 模块和 ShockPod 电击槽
 

波型
指数波或方波

电压,V
10–3,000

电容,µF
10–500 V: 25–3,275 µF,增量为 25 µF;500–3,000 V: 10、25、50 µF

Sample resistance, Ω
20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V

方波计时
10–500 V: 0.05–10 毫秒,增量为 0.05 毫秒,10–100 毫秒脉冲,增量为 1 毫秒,1–10 次脉冲,0.1–10 秒间隔;500–3,000 V: 0.05–5 毫秒,增量为 0.05 毫秒,1–2 次脉冲,5 秒最小间隔

尺寸(宽 x 深 x 高),cm
主单元: 31 x 30 x 14
CE 模块: 31 x 28 x 9
PC 模块: 31 x 28 x 5

重量,kg
主单元: 6.6
CE 模块: 3.1
PC 模块: 1.9

Gene Pulser Xcell 微生物系统 包括主单元、PC 模块和 ShockPod 电击槽
 

波型
指数波或方波

电压,V
10–3,000

电容,µF
10–500 V: 25–3,275 µF,增量为 25 µF;500–3,000 V: 10、25、50 µF

Resistance (parallel), Ω
50–1,000 Ω in 50 Ω increments, plus infinity

Sample resistance, Ω
20 Ω minimum at 200–2,500 V; 600 Ω minimum at 2,500–3,000 V

方波计时
10–500 V: 0.05–10 毫秒,增量为 0.05 毫秒,10–100 毫秒脉冲,增量为 1 毫秒,1–10 次脉冲,0.1–10 秒间隔;500–3,000 V: 0.05–5 毫秒,增量为 0.05 毫秒,1–2 次脉冲,5 秒最小间隔

尺寸(宽 x 深 x 高),cm
主单元: 31 x 30 x 14
CE 模块: 31 x 28 x 9
PC 模块: 31 x 28 x 5

重量,kg
主单元: 6.6
CE 模块: 3.1
PC 模块: 1.9

Gene Pulser Xcell 主单元
 

波型
指数波或方波

电压,V
10–3,000

Capacitance, µF
10, 25, 50

样品电阻
最低 20 Ω(200–2,500 V 时);最低 600 Ω(2,500–3,000 V 时)

方波计时
10–500 V: 0.05–10 毫秒,增量为 0.05 毫秒,10–100 毫秒脉冲,增量为 1 毫秒,1–10 次脉冲,0.1–10 秒间隔;500–3,000 V: 0.05–5 毫秒,增量为 0.05 毫秒,1–2 次脉冲,5 秒最小间隔

尺寸(宽 x 深 x 高),cm
主单元: 31 x 30 x 14
CE 模块: 31 x 28 x 9
PC 模块: 31 x 28 x 5

重量,kg
主单元: 6.6
CE 模块: 3.1
PC 模块: 1.9

配套设备

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) 96-Well Electroporation Plate

1652681

Pkg of 1, multiwell plate for use with the Gene Pulser MXcell electroporation system (#165-2670)

The following replacement is recommended:

  • Gene Pulser Xcell™ 电穿孔系统
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) 24-Well Electroporation Plate

1652682

Pkg of 1, multiwell plate for use with the Gene Pulser MXcell electroporation system (#165-2670)

The following replacement is recommended:

  • Gene Pulser Xcell™ 电穿孔系统
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) 12-Well Electroporation Plate

1652683

Pkg of 1, multiwell plate for use with the Gene Pulser MXcell electroporation system (#165-2670)

The following replacement is recommended:

  • Gene Pulser Xcell™ 电穿孔系统
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.4 cm gap

1652081

Pkg of 5, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.4 cm gap

1652088

Pkg of 50, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) Gene Pulser/MicroPulser Electroporation Cuvettes, 0.4 cm gap

1652091

Pkg of 500, 0.4 cm–gap sterile electroporation cuvette, for use with the Gene Pulser and MicroPulser Systems, for mammalian and other eukaryotic cells

试剂&试剂盒

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) Gene Pulser Electroporation Buffer

1652676

10 x 1.8 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser electroporation system

Gene Pulser MXcell™ 电穿孔系统 | Bio-Rad Laboratories

(停产) Gene Pulser Electroporation Buffer

1652677

30 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser Electroporation System

相关资料

Login Required

Number 描述 Options

5700

Instruction Manual, Gene Pulser MXcell Electroporation Guide, Rev A

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10010739

Instruction Manual, Gene Pulser MXcell Electroporation System, Rev B

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10010740

Instruction Manual, Gene Pulser MXcell Electroporation System Quick Guide, Rev A

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10010741

Instruction Manual, Gene Pulser MXcell Electroporation System Pre Set Protocols, Rev A

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10010742

Instruction Manual, Gene Pulser MXcell Electroporation System Plate, Rev A

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5598

Gene Pulser MXcell Electroporation System Flier, Rev B

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5634

Gene Pulser MXcell Electroporation System Brochure, Rev A

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5858

The Gene Pulser MXcell Electroporation System Provides Reproducible Results in Electroporation Plates and Cuvettes With the Same Protocol, Rev A

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5641

The Gene Pulser MXcell Electroporation System Delivers Consistent Results Required for Optimizing Delivery Protocols, Rev A

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5603

Optimization of Electroporation Using Gene Pulser Electroporation Buffer and the Gene Pulser MXcell Electroporation System, Rev A

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5622

Optimization of Electroporation Conditions With the Gene Pulser MXcell Electroporation System, Rev A

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5686

Optimization of Electroporation Conditions for Jurkat Cells Using the Gene Pulser MXcell Electroporation System, Rev A

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5687

Transfection of Mammalian Cells Using Preset Protocols on the Gene Pulser MXcell Electroporation System, Rev A

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5720

Transfection of Neuroblastoma Cell Lines Using the Gene Pulser MXcell Electroporation System, Rev A

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5704

Electroporation Conditions for Chinese Hamster Ovary Cells Using the Gene Pulser MXcell Electroporation System, Rev A

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5733

Transfection of Chinese Hamster Ovary-Derived DG44 Cells Using the Gene Pulser MXcell Electroporation System, Rev A

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5774

Delivery of siRNA by Electroporation Into Primary Human Neutrophils Using the Gene Pulser MXcell System, Rev A

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5778

Electroporation Parameters for Transfection of HL-60 Leukocytic Cell Line With siRNA Using the Gene Pulser MXcell System, Rev B

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5823

Electroporation of Primary Murine Mast Cells Using the Gene Pulser MXcell Electroporation System, Rev A

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5842

Optimization of Electroporation Conditions for Two Different Burkitt Lymphoma Cell Lines Using the Gene Pulser MXcell System, Rev B

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5860

Analysis of IL-4 Dependent Gene Expression in Namalwa Cells by siRNA Transfection: An Example of Pathway Analysis Using the Gene Pulser MXcell Electroporation System, Rev A

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5904

Transfection of Mouse and Human Embryonic Stem Cells by Electroporation Using the Gene Pulser Mxcell™ System, Rev A

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5935

Electroporation of Smooth Muscle Cells Using the Gene Pulser MXcell Electroporation System, Rev A

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5942

Delivery of siRNA into B-Cell Lymphomas Using the Gene Pulser MXcell Electroporation System, Rev A

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