伯乐MicroRotofor 槽 | Bio-Rad Laboratories

MicroRotofor 槽 | Bio-Rad Laboratories

MicroRotofor 槽使用液相 IEF 在单体溶液中按其 pI 来分离小容量 (2.5 ml) 蛋白质。

描述

MicroRotofor 等电聚焦仪是一种制备型等电聚焦仪,可以根据蛋白质的等电点将少量体积(2.5mL)的蛋白质进行纯化分离。在双向电泳应用中,通过等电点进行蛋白质分离可以去除靶蛋白pI范围外的蛋白,从而提高样品在极窄范围IPG胶条的上样量并提高分离效果。无论是单独使用还是作为一整套纯化方案的一部分,MicroRotofor 都能提供从复杂样品中快速、简单有效的分离和富集目标蛋白质组分的最佳方案。

整个装置易于操作,并具有以下功能:

  • 根据等电点进行液相分离,去除了在等电点范围之外的不感兴趣的蛋白
  • 可对目标蛋白进行高达 10 倍的浓缩
  • 自定义 pH 梯度 — 可使用 Bio-Lyte 两性电解质生成宽或窄范围线性 pH 梯度
  • 快速回收聚焦于 10 个组分中的蛋白
  • 在聚焦过程中,内置的温控系统保证了试验的重复性,并保护样品结构和功能不受破坏
  • 预装的一次性聚焦槽

运行 MicroRotofor 聚焦仪所需的附件包括:1,000 V 电源(例如 PowerPac™ HV)和真空源;另外需要使用 Bio-Lyte® 两性电解质形成 pH 梯度。

Related Products

  • MicroRotofor™ 裂解试剂盒

  • 两性电解质

  • Rotofor® 和 Mini Rotofor 等电聚焦仪

  • PowerPac™ HV 高电压电泳仪电源

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MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Cell Kit

1702800

100/120 V, preparative IEF device, with accessories (high voltage power supply required, #164-5056 sold separately)

The following replacement is recommended:

  • 制备型电泳
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Cell Kit

1702801

220/240 V, preparative IEF device, with accessories (high voltage power supply required, #164-5056 sold separately)

The following replacement is recommended:

  • 制备型电泳
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ System

1702802

100/120 V, preparative IEF device with accessories (#170-2800) with high voltage power supply (#164-5056)

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ System

1702803

220/240 V, preparative IEF device with accessories (#170-2800) with high voltage power supply (#164-5052)

The following replacement is recommended:

  • 制备型电泳
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

配套设备

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) Model EP-1 Econo Pump

7318142

220/240 V, peristaltic pump, two-channel, bidirectional, variable-speed, for use in low-pressure chromatography

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) Model EP-1 Econo™ Pump

7318140

Discontinued Item
Replaced by Standard BioLogic™ LP Low-Pressure Chromatography System (7318300)

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) Model 2110 Fraction Collector

7318122

100/120 V, carousel-type fraction collector, 80 tube capacity, for use in chromatographic separations

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) Model 2110 Fraction Collector

7318120

220/240 V, carousel-type fraction collector, 80 tube capacity, for use in chromatographic separations

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) PowerPac Universal Power Supply

1645070

100–120/220–240 V, power supply for a broad range of electrophoresis and blotting applications, includes power cord

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) Sealing Tape

1702960

Pkg of 1 roll, 1″ x 36 yards, sealing tape, for use with Rotofor and mini Rotofor cell

(停产) MicroRotofor™ Lid

1702855

Pkg of 1, replacement part, for use with MicroRotofor cell

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Vacuum Block O-Ring

1702852

Pkg of 1, replacement part for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Needle Assembly

1702851

Pkg of 1, replacement part for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Harvesting Station

1702850

Pkg of 1, replacement part, includes alignment station, needle assembly, needle holder, for use with MicroRotofor cell

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Syringes

1702836

Pkg of 6, includes 3 each of 3 ml and 10 ml syringes, for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Cleaning Brush

1702835

Pkg of 1, for use with MicroRotofor cell

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Ion Exchange Membrane Assemblies

1702833

Pkg of 1, for use with MicroRotofor cell

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Assembly Tool

1702832

Pkg of 1, plastic riveted block used to prepare tape for sealing the focusing chamber of the MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Anode Assembly

1702829

Pkg of 1 assembly, replacement, for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Electrode Assembly O-Ring/Gasket Kit

1702826

Pkg of 1 set, electrolyte buffer chamber O-ring and gaskets, replacement parts, for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Cathode Assembly

1702822

Pkg of 1 assembly, replacement, for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Focusing Chambers

1702821

Pkg of 3, focusing chamber, for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Sealing Film

1702820

Pkg of 10 sheets, sealing film for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Harvesting Trays

1702810

Pkg of 3, tray for use with MicroRotofor cell

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

MicroRotofor 槽 | Bio-Rad Laboratories

(停产) MicroRotofor™ Starter Kit

1702804

MicroRotofor accessory kit, includes Bio-Lyte® ampholytes, control protein sample, focusing chamber, ion exchange membranes, harvesting tray, syringes

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

相关资料

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Number 描述 Options

5451

First-Dimension Separation With the MicroRotofor Cell Prior to SDS-PAGE and LC-MS/MS Analysis, Rev A

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5349

Fractionation by Liquid-Based Isoelectric Focusing With the MicroRotofor Cell: Improving the Resolution of Low-Abundance Proteins on 2-D Gels (Poster), Rev A

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5344

Fractionation by Liquid-Phase Isoelectric Focusing in the MicroRotofor Cell: Improved Detection of Low-Abundance Proteins, Rev A

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10004399

Instruction Manual, MicroRotofor Cell Starter Kit Manual, Rev A

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10008759

MicroRotofor Cell Tutorial DVD

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5294

MicroRotofor Cell Product Information Sheet

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