A number of assays are available for the determination of protein concentration. The Bio-Rad protein and Quick Start Bradford protein assays are recommended for classroom use.

A number of assays are available for the determination of protein concentration, each with a set of advantages. The Bio-Rad protein and Quick Start™ Bradford protein assays are recommended for classroom use.

Bio-Rad Protein Assay Kit

The simplicity, sensitivity, and color of the Bio-Rad protein assay make it a great vehicle for integrating biology, math, experimental design, and technique. The assay is derived from the Bradford method (1976) and is based on the color change of Coomassie (Brilliant) Blue G-250 dye in direct response to various concentrations of protein. In a typical application, the protein assay reagent is added to a sample and the color change is quantitated with a spectrophotometer or microplate reader. Protein standards provided in this kit allow generation of a standard curve based on the color intensities by known concentrations of protein. The amount of protein in an unknown sample can also be accurately estimated by eye, making this a great low-tech alternative for the classroom. Advantages of the Bio-Rad protein assay kit include:

• Storage up to 1 year at recommended 4°C
• Protein standard included
• Rapid and sensitive
• Classroom alternative to Biuret reagent

Quick Start Bio-Rad Protein Assay Kit

The Quick Start Bradford protein assay quantitates your samples quickly so you can move on with your experiments. The ready-to-use Quick Start assay provides excellent linearity, compatibility with a wide range of reagents, and convenient features such as:

• Prediluted standards
• 1x dye reagent
• Screwcap vials instead of ampoules
• Standard microplate and microassay protocols

References

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.

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(停产) Quick Start Bradford Protein Assay Kit 1

5000201EDU

Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard (5 x 2 mg/ml); 200 standard assays or 4,000 microplate assays; education use only

(停产) Quick Start Bradford Protein Assay Kit 2

5000202EDU

Colorimetric assay kit, includes 1x dye reagent (1 L), BSA standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml); education use only

(停产) Quick Start Bradford Protein Assay Kit 3

5000203EDU

Colorimetric assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard (5 x 2 mg/ml); education use only

(停产) Quick Start Bradford Protein Assay Kit 4

5000204EDU

Colorimetric protein assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml); education use only

(停产) Bio-Rad Protein Assay Kit I

5000001EDU

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine γ-globulin standard; for 440 standard assays or 2,200 microplate assays; education use only

(停产) Bio-Rad Protein Assay Kit II

5000002EDU

Colorimetric assay kit, includes 450 ml dye reagent concentrate, bovine serum albumin standard; for 440 standard assays or 2,200 microplate assays; education use only

(停产) Bio-Rad Protein Assay Dye Reagent Concentrate

5000006EDU

450 ml, colorimetric assay dye, concentrate, for use with Bio-Rad protein assay kits I and II (#500-0001EDU, 500-0002EDU); education use only

(停产) Protein Standard II

5000007EDU

Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted); education use only

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