Based on the Bio-Rad Quick Start Bradford protein assay, this kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments.

Based on the Bio-Rad Quick Start™ Bradford protein assay, the Got Protein™ Kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments. In this laboratory activity, students use absorbance values from a set of protein samples with known concentrations to create a standard curve on linear graph paper. Protein concentrations of their test samples, such as milk, egg, sports drink, or saliva, can then be extrapolated by hand or plotted using a graphing utility such as Microsoft Excel software.

Features and Benefits

• Inquiry-based activity
• Integrates biology, chemistry, and physics in relation to protein structure and function

With this kit, students are able to:

• Measure protein concentrations
• Apply Beer’s law
• Learn to use a spectrophotometer
• Learn to use a micropipet

How It Works

The Bradford method is based on the color development formed when the dye Coomassie Blue G–250 binds to protein. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye or quantitatively with a spectrophotometer.

More Information

• One 45 min laboratory activity
• Provides sufficient materials for eight student workstations, up to four students per workstation.

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(停产) Got Protein? Kit

1662900EDU

Introductory proteomics classroom study kit, includes Quick Start Bradford protein assay kit 4 (#500-0204EDU), 10x PBS, curriculum, and more, for 320 students; education use only

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