The EXQuest spot cutter is a precision instrument that accurately locates and excises protein bands or spots from 1-D and 2-D gels or blots and loads them into multiwell microplates or 96-tube racks.
描述
Discontinued Product The ExQuest™ Spot Cutter has been discontinued. We will continue to provide support for ExQuest owners until March, 2020.
The EXQuest™ spot cutter is a precision instrument that accurately locates and excises protein bands or spots from 1-D and 2-D gels or blots. It then loads them into 96- and 384-well microplates or 96-tube racks for downstream processing and analysis. The EXQuest spot cutter is designed to be both robust in construction and simple to operate, reducing manual labor and improving throughput of protein spots for analysis.
The EXQuest spot cutter is a fully integrated system controlled through PDQuest™ 2-D analysis software or Quantity One® 1-D analysis software. It consists of an enclosure, an imaging system, a fluidics system, robotics, sensors, a cutting head, a gel tray, a microplate rack, and a wash station.
Versatile Excision Capability
The EXQuest spot cutter allows use of any common proteome separation and staining methods:
Freestanding 2-D and 1-D SDS-PAGE gels
Plastic- or glass-backed 2-D and 1-D gels
PVDF and nitrocellulose membrane blots
Gels or membranes stained for proteins with visible stains (such as silver and Coomassie blue stains) or fluorescent stains (such as Flamingo™ fluorescent gel stain and SYPRO Ruby protein stain)
Big Performance on the Bench
Accommodates any commercially available large or mini format gel or PVDF membrane blot in a small bench footprint
PDQuest software automatically selects spots in order from lowest to highest amount of protein, minimizing the chance of carryover contamination from spot to spot
Quantity One software provides convenient tools for 1–D gel cutting
Cutting tip produces precise cuts without damage or distortion on gels or PVDF membranes
Cuts up to 4 gels at a time at up to 600 spots per hour
System accuracy of ±0.1 mm ensures accurate excision of even small, closely grouped spots
Error-free data capture from image analysis to mass spectrometry
Built-in wash wells provide customized tip washing between cuts for extra contamination control
Quick-change settings to switch between UV and visible light modes
Compatible with ProteomeWorks™ and ProteomeWorks Plus spot cutters
Related Technologies
Imaging and Analysis of 2-D Electrophoresis Gels
2-D Electrophoresis
2D Protein Spot Excision & Detection
Types of Gel Analysis Software
Related Products
考马斯染料
常规电泳染色和跟踪染料
Silver Stain Plus 试剂盒
Quantity One 1-D 分析软件
技术指标
系统精度
±0.1 mm (100 µm)
切割速度
600 spots per hour
切取平台
Accommodates one large gel (e.g., PROTEAN® Plus handcast gels) or up to four small gels (e.g., Criterion™ gels) without the need for trimming
切取率
>99.5% spot pickup on first cut
CCD镜头
1,600 x 1,200 imaging pixel resolution, 100 µm resolution, 12-bit, cooled to 0°C
切取头内径
1.5 mm, 1.0 mm, and optional membrane cutting tip
切割对象
Backed or free gels 1-D and 2-D gels 0.5–2.0 mm thick; visible light applications (e.g., Coomassie blue, silver stain); UV light applications (e.g., Flamingo™, SYPRO Ruby); PVDF and nitrocellulose membrane blots
工作温度
15–30°C
工作湿度范围
12–85%, noncondensing
体积（不含计算机）（宽 x 深 x 高）
91 x 69 x 66 cm (35.8 x 27.2 x 26.0″). Requires 38 cm clearance above the front of the instrument and 25 cm clearance on left side of instrument
Weight
Instrument only, 76 kg (168 lb)
最低计算机配置要求（不含计算机）
Microsoft Windows 2000 or XP operating system (1.2 GHz), 2.1 GB hard drive, 512 MB RAM, screen resolution 1,024 x 768, 2 MB video RAM
连接类型
USB
订购
items
Use the filters below to refine results!
(停产) EXQuest Spot Cutter With PC
1657201
Gel excision instrument, includes PC, enclosure, imaging system, fluidics system, robotics, sensors, cutting head, gel tray, microplate track, wash station
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
配套设备
(停产) Cutting Tip
1657202
Pkg of 1, 1.0 mm cutting tip, for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Cutting Tip
1657203
Pkg of 1, 1.5 mm cutting tip, for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Glass Bottle
1657204
Pkg of 1, 1 L glass bottle for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Calibration Pucks
1657205
Pkg of 10, calibration puck for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Membrane Cutting Head
1657206
Pkg of 1, cutting head with 1.0 mm tip, for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Membrane Cutting Tip
1657207
Pkg of 1, 1.0 mm cutting tip, for use with membrane cutting head (#165-7206) on EXQuest spot cutter (#165-7200, #165-7201)
(停产) Gel Cutting Sheets
1657208
Pkg of 15, gel cutting sheet, for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Gel Holding Clips
1657209
Pkg of 2, gel holding clip for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Calibration Target
1657210
Pkg of 1, calibration target for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Camera Target
1657211
Pkg of 1, camera target for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Micro Tubes
1657212
Pkg of 20, 1.5 ml, micro tube for use with EXQuest spot cutter (#165-7200, #165-7201)
(停产) Bottle Holder
1657214
Pkg of 1, bottle holder for use with EXQuest spot cutter (#165-7200, #165-7201)
(停产) Gel Tray
1657215
Pkg of 1, gel tray for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Transilluminator Lamp
1657216
Pkg of 1, transilluminator lamp for use with EXQuest spot cutter (#165-7200, #165-7201)
(停产) Rounded-Bottomed Microplates
1657217
Pkg of 20, 96-well microplate, for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Ferrule
1657218
Pkg of 10, 10-32, 1/16″ OD, ferrule for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Bar Code Reader
1657219
Pkg of 1, bar code reader for use with EXQuest spot cutter (#165-7200, #165-7201)
本产品不提供在线销售。欲了解更多信息，请联系客户服务。
(停产) Microplate Holder
1657220
Pkg of 1, microplate holder for use with EXQuest spot cutter (#165-7200, #165-7201)
(停产) Quantity One® 1-D Analysis Software
1709600
1-user license, PC or Mac, image acquisition and analysis software for use with Bio-Rad imaging systems
