伯乐Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

Profinia 蛋白质纯化仪器结构紧凑,简单易用。 它以一套完整的蛋白纯化和脱盐系统取代了传统的重力流技术。

描述

Profinia 蛋白纯化系统设计紧凑,方便使用。每一个组件都标注清晰以便快速安装。缓冲液部分便于用户放置溶液 — 仪器上的位置编号与运行图和预包装试剂上的标签相匹配。样品部分可以依次进行两种样品的纯化。这两部分都含有吸管用于样品和缓冲液的吸取。预装柱部分提供了两步纯化的能力,而且使用Luar的接头方便预装柱的连接。废液收集瓶和稀释液瓶分别位于仪器的两侧。组分收集和样品部分的独特设计使标准收集管的安装快速而简便。仪器的中央部分能够提供方便的存储,USB 接口可轻松地将数据导出。

该仪器有一个很大的触摸屏式的用户界面,用户可以选择指尖或手写笔进行界面的切换。直观友好的帮助功能可以引导用户使用预设的纯化方法进行启动和运行后操作。操作方法和缓冲液设置还包括了清洗步骤,能自动维护系统并准备下一次纯化的预装柱,以确保 Profinia 蛋白纯化系统能维持最佳的运行状态。这些功能的组合,使用户能快速启动,然后即可离开,直到 30 分钟再返回直接获取结果。

  • 优化的方法、预装柱和缓冲液试剂盒能减少进行方法、故障探查和试剂准备所花费的时间
  • 全自动系统泵、紫外和电导检测器以及程序化的清洗方法能保障结果的重复性
  • 预设的His肽标记、GST标记及其脱盐/缓冲液交换的方法可以获得纯度、得率都优于其他方法的目标蛋白,而用户只需要花费很短的时间进行样品的准备即可
  • 全自动紫外峰值检测器可以将预装柱上的洗脱的蛋白自动进行收集,整个过程无需人工干预
  • 方便操作的组分收集器和样品管能容纳 15 或 50ml 的圆锥形收集管
  • 台式或低温操作设计使您能控制纯化条件,可选的制冷附件能使样品和组分在台面上也能保持低温
  • 可选的pH计可以实时监测运行状况。

Related Technologies

  • Affinity Purification of Tagged Recombinant Proteins

Related Products

  • Profinia 蛋白质纯化系统附件

  • BioLogic DuoFlow 10 系统

  • Profinia 蛋白质纯化系统软件

  • Profinia 蛋白质纯化系统试剂盒

技术指标

操作限制
 

流速范围
0.2–20 ml/min

最大背压
45 psi/3.4 bar

操作温度范围
4–30ºC

检测
 

紫外吸收波长
280 nm

电导范围
0–500 ms/cm

pH 计(可选)PH 范围
1–14

硬件
15 或 50ml 收集管,还可通过管线连接更大体积的外接收集器

收集容器
旋转液流选择阀,最多可选择 8 种溶液

压力
传感器可监测系统压力

溶剂兼容性
所有层析过程常见溶液

数据
 

输出 USB
USB 闪存盘或者通过 USB 线连接电脑输出

报告软件(可选)
Windows 2000 或者 XP,层析数据实时展示,报告和分析或者保存运行数据

用户界面
触摸屏 115 x 86 mm;通过手写笔或者手指选择方法,样品和预装柱

可选附件(单独出售)
样品和组分冷却组件,脱盐样品环,pH 计,Profinia 软件

物理规格
 

Dimensions (W x D x H)
58 x 33 x 67 cm

订购

items

Use the filters below to refine results!

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia™ Instrument With Accessory Kit and Native IMAC Starter Kit

6201005

Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia™ Instrument With Accessory Kit and GST Starter Kit

6201006

Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia™ Protein Purification System With Native IMAC Starter Kit

6201010

Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia™ Protein Purification System With GST Starter Kit

6201011

Discontinued item – replaced by the NGC™ Medium-Pressure System or BioLogic™ Low-Pressure System.

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Protein Purification System With Computer and Native IMAC Starter Kit

6201015

100–240 V, automated chromatography system, includes Profinia instrument and software, computer, Profinia instrument accessory kit (#620-0410) and Profinia native IMAC starter kit (#620-0229)

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Protein Purification System With Computer and GST Starter Kit

6201016

100–240 V, automated chromatography system, includes Profinia instrument and software, computer, Profinia instrument accessory kit (#620-0410) and Profinia GST starter kit (#620-0230)

配套设备

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Software

6200010

PC, version 2, software for monitoring and reporting data from the Profinia protein purification instrument, CD-ROM, includes USB cable

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Instrument Cooling Accessory

6200401

Pkg of 2, cooling unit for sample loading and collection, for use with the Profinia protein purification instrument

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia pH Monitor Kit

6200411

Inline pH monitor for use with the Profinia protein purification instrument, includes pH electrode, flow cell, mounting accessories

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Desalting Sample Loop

6200402

Pkg of 1, 2 ml, desalting cartridge accessory for use with the Profinia protein purification system, includes 10 ml syringe and fittings

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Desalting Sample Loop

6200403

Pkg of 1, 10 ml, desalting cartridge accessory for use with the Profinia protein purification system, includes 10 ml syringe and fittings

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Instrument Inline Filter Pack

6200404

Pkg of 12 filters, replacement filters to reduce line clogging and minimize high backpressure on the Profinia protein purification instrument

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Sipper Tube Replacement Kit

6200405

Pkg of 10 pieces, precut 13.5 cm (5.3″) polypropylene tubing for buffer and sample inlet compartments on the Profinia protein purification instrument

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Instrument Accessory Kit

6200410

Accessory kit for the Profinia protein purification instrument, includes cleaning tray, inline filter pack, sample lids, bottle starter pack, waste/diluent bottle set

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bottle Starter Pack

6200231

Pkg of 8, includes 4 x 125 ml buffer bottles, 4 x 250 ml buffer bottles, 8 buffer bottle lids; for use with the Profinia protein purification system

试剂&试剂盒

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia IMAC Purification Kit

6200225

Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 2 each of 1 ml IMAC and 10 ml desalting cartridges

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia IMAC Purification Kit

6200235

Kit for use with the Profinia protein purification system, includes 1 each of 5 ml IMAC and 50 ml desalting cartridges, 2 Profinia IMAC buffer kits (#620-0221)

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia GST Purification Kit

6200226

Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 2 each of 1 ml GST and 10 ml desalting cartridges

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia GST Purification Kit

6200236

Kit for use with the Profinia protein purification system, includes 1 each of 5 ml GST and 50 ml desalting cartridges, 2 Profinia GST buffer kits (#620-0223)

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Desalting Purification Kit

6200228

Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia Desalting Purification Kit

6200238

Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia IMAC Buffer Kit

6200221

Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia GST Buffer Kit

6200223

Buffers, solutions, and glutathione sufficient for 10 applications, for use with the Profinia protein purification system

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Desalting and Cartridge Cleaning Buffer Kit

6200224

Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia IMAC Starter Kit

6200229

Kit for use with the Profinia protein purification system, includes Profinia IMAC buffer kit (#620-0221), 1 each of 1 ml IMAC and 10 ml desalting cartridges, E. coli lysate

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Profinia GST Starter Kit

6200230

Kit for use with the Profinia protein purification system, includes Profinia GST buffer kit (#620-0223), 1 each of 1 ml GST and 10 ml desalting cartridges, E. coli lysate, glutathione

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) His Antibody

6200203

Pkg of 1 vial, 1 mg/ml, monoclonal antibody against histidine (His)-tagged proteins, for use with the Profinia protein purification system

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) GST Antibody

6200204

Pkg of 1 vial, 1 mg/ml, monoclonal antibody against GST-tagged proteins, for use with the Profinia protein purification system

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) His and GST Purification E. coli Control Lysate

6200233

Lyophilized, dual-tagged 51 kD protein for system setup and initial purification, for use with the Profinia protein purification system

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Glutathione Pack

6200202

1.23 g, powdered reagent, for denaturing GST applications on the Profinia protein purification system

预装柱

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity IMAC Cartridges

7324610

Pkg of 5, 1 ml, prepacked Ni-charged column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity IMAC Columns, Ni-charged, 5 x 1 ml ( 12009298 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity IMAC Cartridge

7324612

Pkg of 1, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity IMAC Column, Ni-charged, 1 x 5 ml ( 12009299 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity IMAC Cartridges

7324614

Pkg of 5, 5 ml, prepacked Ni-charged column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity IMAC Columns, Ni-charged, 5 x 5 ml ( 12009300 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity GST Cartridges

7324620

Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity GST Columns, 5 x 1 ml ( 12009295 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity GST Cartridge

7324622

Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity GST Column, 1 x 5 ml ( 12009296 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity GST Cartridges

7324624

Pkg of 5, 5 ml, prepacked affinity columns, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Profinity GST Columns, 5 x 5 ml ( 12009297 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity eXact Cartridges

7324646

Pkg of 2, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity eXact Cartridges

7324647

Pkg of 4, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Profinity eXact Cartridge

7324648

Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic chromatography and Profinia systems

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Affi-Prep Protein A Cartridges

7324600

Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Affi-Prep Protein A Columns, 5 x 1 ml ( 12009236 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale Mini Affi-Prep Protein A Cartridge

7324602

Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

The following replacement is recommended:

  • EconoFit Affi-Prep Protein A Column, 1 x 5 ml ( 12009237 )
本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 10 ml

7325304

Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 50 ml

7325312

Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Profinia 蛋白质纯化仪器 | Bio-Rad Laboratories

(停产) Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 50 ml

7325314

Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

相关资料

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Number 描述 Options

10006036

Instruction Manual, Profinia Protein Purification System, Rev F

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10015932

Profinia™ Protein Purification System FAQs, Rev B

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10015931

Understanding Chromatograms Generated by the Profinia Protein Purification System Reference Guide, Rev B

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10006081

GST Antibody Instruction Manual, Rev C

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5514

Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A

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5513

Automated Purification of a GST-Tagged Protein With the Profinia Protein Purification System: Comparison to Manual Protein Purification Using Commercial Kits, Rev A

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5539

Automated Desalting of Proteins With the Profinia Protein Purification System: Comparison to Manual Desalting by Dialysis, Rev A

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5712

The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A

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5726

Profinia Protein A and G methods Product Information Sheet, Rev A

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5725

Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A

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5741

Profinia Buffers for Maltose Binding Protein (MBP) Chromatography Product Information Sheet, Rev A

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5742

Profinity eXact Purification Methods on the Profinia System, Rev. A

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5744

Profinia Buffers for Strep-Tag Protein Chromatography Product Information Sheet, Rev A

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5766

Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A

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5573

The Profinia Protein Purification System: An Automated Solution for Purifying Affinity-Tagged Proteins, Rev A

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5541

Profinia Protein Purification System Product Information Sheet, Rev A

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5510

Profinia Protein Purification System Brochure, Rev A

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5701

Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B

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5926

Modular Protein Purification, Rev A

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