伯乐荧光蛋白染色剂 | Bio-Rad Laboratories

荧光蛋白染色剂 | Bio-Rad Laboratories

如果将荧光染色与相应的成像器组合起来,这将是一种比 Coomassie 和银染更加优越的蛋白质分析方法。 Bio-Rad 提供了两种类型的荧光染料。

描述

如果将荧光蛋白质凝胶染色与相应的图像仪组合起来,将是一种比 Coomassie 和银染更好的蛋白分析方法。 荧光凝胶染料具有宽广的线性范围、更高的灵敏度以及质谱分析兼容性等优势。 Bio-Rad 提供三种类型的荧光蛋白凝胶染料,每种均根据您的图像仪要求进行了优化:

Oriole™ 荧光凝胶染料 — 一种易于使用、快速、灵敏、高度线性的蛋白凝胶染料,用于查看及定量测定 SDS-PAGE 凝胶中的蛋白质。 简单的实验方案只需要执行一步法染色过程而无需提前修复。 由于不需要脱色,因此在不到 2 小时内即可准确显示和定量蛋白样品。 Oriole 的激发和发射特征使其成为紫外图像仪的理想选择。

Flamingo™ 荧光凝胶染料 — 这种 10x 溶液中含有一种新型染料,在与变性蛋白结合时会发出荧光。 这种易于使用的凝胶染料可用于垂直电泳和双向电泳应用,并能检测低至 0.5 ng 的蛋白。 染色过程是简单的两步实验方案,可在短短的 5 小时内完成。 这些步骤对时间不敏感并且不需要脱色。 可在 2–8°C 下的黑暗环境中储存染色凝胶 6 个月之久,且成像灵敏度不会明显降低。 Flamingo 荧光凝胶染料与质谱分析相容。 使用 Flamingo 荧光凝胶染料染色的凝胶还可以使用各种荧光成像系统显示出来,但这种凝胶特别针对基于激光的荧光扫描仪(如 Molecular Imager® PharosFX™ 系统)进行了优化。

SYPRO Ruby 蛋白凝胶染料 — 即用型 SYPRO Ruby 蛋白凝胶染料是适用于双向电泳的使用最广泛的凝胶染料,与质谱法以及基于 Edman 的测序应用相容。 可使用紫外或蓝光透射仪或激光扫描仪显示它。

Related Technologies

  • 2D Protein Spot Excision & Detection

  • 2-D Electrophoresis and Analysis

  • Staining and Visualization of Proteins After 2-D Electrophoresis

Related Products

  • 考马斯染料

  • 银染

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荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Oriole Fluorescent Gel Stain

1610495

200 ml, 1x Oriole fluorescent gel stain solution

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Oriole Fluorescent Gel Stain

1610496

1 L, 1x Oriole fluorescent gel stain solution

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Oriole Fluorescent Gel Stain, Kit

1610497

5 L kit, materials sufficient for 5 L of 1x Oriole fluorescent stain solution

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Flamingo™ Fluorescent Protein Gel Stain, 20 ml

1610490

20 ml, 10x Flamingo Fluorescent Gel Stain solution

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Flamingo™ Fluorescent Protein Gel Stain, 100 ml

1610491

100 ml, 10x Flamingo Fluorescent Gel Stain solution

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Flamingo™ Fluorescent Protein Gel Stain, 500 ml

1610492

500 ml, 10x Flamingo Fluorescent Gel Stain solution

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) SYPRO Ruby Protein Gel Stain, 200 ml

1703126

200 ml, 1x solution, ready-to-use fluorescent gel stain

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) SYPRO Ruby Protein Gel Stain, 1 L

1703125

1 L, 1x solution, ready-to-use fluorescent gel stain

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) SYPRO Ruby Protein Gel Stain, 5 L

1703138

5 L, 1x solution, ready-to-use fluorescent gel stain

配套设备

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Criterion Staining/Blotting Trays

3459921

Pkg of 2, gel staining tray, with lids

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Criterion Staining/Blotting Trays

3459920

Pkg of 12, gel staining trays, with lids

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Storage Box

1653430

Pkg of 1, small box for storing gels, holds up to 4 gels on small staining trays

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Storage Box

1653429

Pkg of 1, large box for storing gels, holds up to 4 gels on large staining trays

荧光蛋白染色剂 | Bio-Rad Laboratories

(停产) Dodeca™ Gel Stainer, small

1653401

100–240 V, small gel staining device, includes 13 trays, 12 Criterion tray attachments, shaking rack, solution tank, lid with motor, shaker control, gel clip

相关资料

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Number 描述 Options

5991

Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A

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SYPRO Ruby Protein Gel Stain Flier, Rev E

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Sensitivity and Protein-to-Protein Consistency of Flamingo Fluorescent Gel Stain Compared to Other Fluorescent Stains, Rev A

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Flamingo Fluorescent Gel Stain Product Information Sheet, Rev A

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Instruction Manual, Flamingo Fluorescent Gel Stain, Rev A

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Instruction Manual, SYPRO Ruby Protein Stains, Rev B

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Oriole Fluorescent Gel Stain PIS, Rev A

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Comparison of SYPRO Ruby and Flamingo Fluorescent Gel Stains With Respect to Compatibility With Mass Spectrometry, Rev A

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Oriole Fluorescent Gel Stain: Characterization and Comparison with SYPRO Ruby Gel Stain, Rev A.

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Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide

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