伯乐PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit | Bio-Rad Laboratories

The PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit provides reproducible, quantitative results in wastewater testing for SARS-CoV-2, the virus caused by COVID-19.

描述

The PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit provides reproducible, quantitative results in wastewater testing for SARS-CoV-2, the virus caused by COVID-19.

The PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit, based on the U.S. CDC and WHO RT-PCR primers for SARS-CoV-2 detection, allows for the detection of N2 and E genes in SARS-CoV-2. It also includes an assay for the Murine Coronavirus or Murine Hepatitis Virus (MHV), an enveloped, single-stranded RNA virus. MHV serves as a matrix recovery control, enabling estimation of the virus lost during sample processing. The test allows for all three targets to be detected in a single well using the kit. The kit also contains reagents sufficient for 200 reactions, and there is an automated software tool available for analysis.

Features and Benefits

Addresses an unmet need in COVID surveillance — the ability to test millions of people with sufficient lead time to take action
Quantitative without requiring a standard curve
More inhibitor-resistant compared to qPCR
Reproducible results across runs, reagent lots, and instruments
Single-well multiplex test, or two two-well test if using fecal indicator
Turnaround time <8 hours from sample to answer
Validated on the QX200 ddPCR Platforms, compatible with QX ONE ddPCR Platform
Aligned to CDC guidelines on wastewater testing
Multiple viral targets for more robust assay performance
Automatic analysis with the PREvalence Wastewater Automated Analysis Application

Applications and Uses

Detecting SARS-CoV-2 virus in wastewater

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(停产) PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit

12015402

200 reactions, includes ddPCR Triplex Assay (SARS-CoV-2 N2, E, and MHV), Supermix for One-Step RT ddPCR Advanced Mix for Probes, RT for One-Step RT ddPCR Advanced Mix for Probes, DTT, and positive control

(停产) PREvalence ddPCR crAssphage Fecal Indicator Assay

12015961

100 reactions, includes ddPCR crAssphage Fecal Indicator Assay used with the PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit (12015402)

(停产) PREvalence ddPCR PMMoV Fecal Indicator Assay

12015962

100 reactions, includes ddPCR PMMoV Fecal Indicator Assay used with the PREvalence ddPCR SARS-CoV-2 Wastewater Quantification Kit (12015402)

Other

(停产) ddPCR™ Droplet Reader Oil

1863004

2 L (2 x 1 L), oil for use with droplet reader in the QX200™/QX100™ Droplet Digital™ PCR Systems

(停产) DG32 Automated Droplet Generator Cartridges

1864108

Pkg of 30, cartridges for use with the Automated Droplet Generator in the QX200 AutoDG Droplet Digital PCR System

(停产) Pipet Tips for the AutoDG™ System

1864120

Pkg of 20, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

(停产) Pipet Tip Waste Bins for the AutoDG™ System

1864125

Pkg of 10, pipet tip waste bins for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

(停产) Automated Droplet Generation Oil for Probes

1864110

140 ml, oil for use with the Automated Droplet Generator for probe-based detection with the QX200 AutoDG Droplet Digital PCR System

(停产) PCR Plate Heat Seal, foil, pierceable

1814040

Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

(停产) Microseal® 'F' PCR Plate Seal, foil, pierceable

MSF1001

Pkg of 100, aluminized foil seal for PCR plates, pierceable

(停产) DG8™ Cartridges for QX200™/QX100™ Droplet Generator

1864008

Pkg of 24, cartridges for use with the QX200™/QX100™ Droplet Generator

(停产) DG8™ Gaskets for QX200™/QX100™ Droplet Generator

1863009

Pkg of 24, gaskets for use with QX200™/QX100™ Droplet Generator

(停产) DG8 Cartridge Holder

1863051

Pkg of 1, cartridge holder, positions and holds the cartridge in the QX200 Droplet Generator

(停产) Droplet Generation Oil for Probes

1863005

70 ml (10 x 7 ml), oil for use with droplet generator in the QX200/QX100 Droplet Digital™ PCR Systems

Instrument

(停产) QX200 Droplet Reader

1864003

Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200 Droplet Digital™ System

(停产) Automated Droplet Generator

1864101

AutoDG Instrument for automated droplet generation of up to 96 samples, for use with the QX200/QX100; Droplet Digital PCR Systems

(停产) QX200™ Droplet Generator

1864002

Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

(停产) C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module

1851197

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

(停产) PX1 PCR Plate Sealer

1814000

PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

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QX Manager Software Standard Edition, Version 1.2

QX-MANAGER-V.1.2-STD

QX Manager Software Standard Edition, Version 1.2

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EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

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PREvalence Wastewater Automated Analysis Application

Covid_WW_Interpretive_Application_1.0.0.6

Automate analysis with the PREvalence Wastewater Automated Analysis Application