伯乐Cell Lysis RT-qPCR Kits | Bio-Rad Laboratories

SingleShot Cell Lysis RT-qPCR Kits allow RT-qPCR to be performed directly from cultured cells without RNA purification. Kits are available for both one-step and two-step RT-qPCR with SYBR Green and probe chemist

描述

Cell Lysis RT-qPCR Kits | Bio-Rad Laboratories

SingleShot Cell Lysis RT-qPCR Kits provide a complete and fast solution for the generation of lysates from cell cultures, optimized for downstream one step or two step quantitative PCR (qPCR) reactions, without an RNA purification step.

Kits are available in SYBR Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option.

All kits offer the following key benefits:

Cell lysate from 10 to 100,000 cells ready in 20 minutes
Complete removal of genomic DNA while preserving RNA integrity
Optimal accuracy and linear dynamic range of qPCR data across genes
Results comparable to using purified RNA, including dynamic range
Validated with PrimePCR Assays and Panels
SingleShot RNA Control ensures optimal cell and lysate inputs

Choose the Right Kit for Your Needs

SingleShot Kit	Features	Product Options
Cell Lysis One-Step RT-qPCR Kits	Cell lysis, reverse transcription, and qPCR All one-step RT-qPCR reagents included No RNA purification needed	SYBR Green 100 reactions (1725095) Probes 100 reactions (1725070)
Cell Lysis Two-Step RT-qPCR Kits	Cell lysis, reverse transcription, and qPCR All two-step RT-qPCR reagents included No RNA purification needed	SYBR Green 100 reactions (1725085) Probes 100 reactions (1725090)
Cell Lysis Kits	Cell lysis for RT-qPCR RT-qPCR reagents sold separately No RNA purification needed Validated with Reliance One-Step Multiplex Supermix and iTaq Universal One-Step RT-qPCR Kits	100 reactions (1725080) 500 reactions (1725081)

Simple Workflow

Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution.

Grow cells to desired density, transfer up to 10⁵ cells to wells of a PCR plate, add lysis solution, and incubate at room temperature for 10 minutes.

Heat for 5 minutes at 37°C and then for 5 minutes at 75°C. The lysate is ready for RT-qPCR or can be stored for future use.

Cell Lysis RT-qPCR Kits | Bio-Rad Laboratories

Cell Lines Validated for Use with the SingleShot Cell Lysis RT-qPCR Kits

All SingleShot Cell Lysis RT-qPCR Kits have been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line	Property	Species
A549	Adherent	H. sapiens, lung carcinoma
HeLa	Adherent	H. sapiens, cervical adenocarcinoma
HepG2	Adherent	H. sapiens, liver carcinoma
NIH/3T3	Adherent	M. musculus, embryonic fibroblast
MCF-7	Adherent	H. sapiens, epithelial, adenocarcinoma
NCI-H1975	Adherent	H. sapiens, lung adenocarcinoma
K-562	Suspension	H. sapiens, chronic myelogenous leukemia
HEK 293	Suspension	H. sapiens, kidney
Jurkat	Suspension	H. sapiens, T lymphocyte
PC-12	Suspension	R. norvegicus, pheochromocytoma, adrenal
HepRG	Primary-like	H. sapiens, hepatocytes

Related Categories

RNA Isolation Kits
Reverse Transcription Kits
Real-time PCR Supermixes

Supporting Documentation

SingleShot Probes Kit Instruction Manual
SingleShot SYBR Green Kit Instruction Manual
SingleShot SYBR Green One-Step Kit Instruction Manual
SingleShot Probes One-Step Kit Instruction Manual
SingleShot Cell Lysis Kit Instruction Manual

Online Resources

Gene Expression Analysis
Nucleic Acid Extraction and Purification
qPCR/Real-Time PCR
qPCR Assay Design and Optimization Protocols
qPCR Data Analysis
Troubleshooting Real-Time PCR Reactions

技术指标

在 4°C 下存储

6 months:
Lysis buffer

3 months:
iTaq™ Universal One-Step Reaction Mix
SsoAdvanced™ Universal Supermix

在 –20°C 下存储

All kit components are stable for 12 months

订购

items

Use the filters below to refine results!

(停产) SingleShot Cell Lysis Kit, 100 x 50 µl rxns

1725080

Pkg of 1, kit includes 5 ml (1 x 5 ml) of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions.

(停产) SingleShot Cell Lysis Kit, 500 x 50 µl rxns

1725081

Pkg of 1, kit includes 25 ml (5 x 5 ml) of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions

(停产) SingleShot™ SYBR^® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725085

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR^® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

(停产) SingleShot™ Probes Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725090

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

(停产) SingleShot™ SYBR^® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725095

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR^® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

(停产) SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725070

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal Probes One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

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