伯乐Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

基于 AP 的比色试剂盒利用 5-溴-4-氯-3-吲哚基磷酸酯/硝基蓝四氮唑 (BCIP/NBT),这种物质可与 AP 发生化学反应以形成有色沉淀。 可以检测只有 100 pg 的蛋白质。

描述

HRP、AP 等酶可把某些底物转变为有色沉淀。当沉淀在印迹膜上聚集,就产生了可见的有色信号。反应可监控,当观察到目标信号超过背景时,即停止反应。比色检测比以胶片为基础的化学发光检测操作更简单; 然而,此方法的单一的最终结果不能产生化学发光法所拥有的多次曝光能力。与放射性或化学发光检测相比,一般认为比色检测是一种中度灵敏的方法。

Bio-Rad 基于 AP 的比色试剂盒利用 5-溴-4-氯-3-吲哚基磷酸酯/硝基蓝四氮唑 (BCIP/NBT),这种物质可与碱性磷酸酶发生反应形成有色沉淀以便检测。 Immun-Blot AP 测定试剂盒包括比色检测所必需的所有试剂,最多可检测 100 pg 的蛋白质。 为获得较高灵敏度,Immun-Blot 扩增型 AP 测定试剂盒使用生物素-链亲和素共轭系统来扩增比色信号,可以检测低至 10 pg 的蛋白质。

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Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) Immun-Blot Goat Anti-Rabbit IgG (H + L)-AP Assay Kit

1706460

Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) Immun-Blot Goat Anti-Mouse IgG (H + L)-AP Assay Kit

1706461

Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) Immun-Blot Goat Anti-Human IgG (H + L)-AP Assay Kit

1706462

Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) Blotting-Grade Biotinylated AP Conjugate

1706403

1 ml, alkaline phosphatase (AP) secondary antibody conjugate

本产品不提供在线销售。欲了解更多信息,请联系客户服务。

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) Blotting-Grade Blocker

1706404

300 g, nonfat dry milk for western blotting applications

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) AP Conjugate Substrate Kit

1706432

Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) AP Color Development Reagent

1706539

300 mg, 5-bromo-4-chloro-3-indolyl phosphate (BCIP), colorimetric substrate, for purple color development; requires AP color development reagent (#170-6532)

本产品不再供货。欲了解更多关于更换产品的信息,请联系移动电话

Immun-Blot® AP 比色试剂盒 | Bio-Rad Laboratories

(停产) AP Color Development Reagent

1706532

600 mg, nitroblue tetrazolium (NBT), colorimetric substrate, for purple color development; requires AP color development reagent (#170-6539)

本产品不再供货。欲了解更多关于更换产品的信息,请联系移动电话

相关资料

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2032

Western Blotting Detection Reagents Brochure, Rev F

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LIT169

Instruction Manual, Alkaline Phosphatase Conjugate Substrate Kit, Rev E

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LIT171

Instruction Manual, Immun-Blot® Kit

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