伯乐Nuvia cPrime Media | Bio-Rad Laboratories

Nuvia cPrime Media | Bio-Rad Laboratories

Nuvia cPrime mixed-mode media is designed for versatile capture and high recovery across a wide range of salt concentrations and pH in process-scale purification processes for biotherapeutic applications.

描述

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Nuvia cPrime hydrophobic cation exchange media are a new addition to Bio-Rad’s family of mixed-mode purification products. These media are designed for process-scale purification of a wide variety of therapeutic proteins. Nuvia cPrime media’s unique selectivity allows method developers to use hydrophobic and cation exchange interaction modes to achieve effective purification. More importantly, the media have a large design space for binding and elution, allowing for the development of highly robust methods in a commercial manufacturing setting. Nuvia cPrime media are built on a rigid, mechanically and chemically stable macroporous base matrix with a particle size optimized to deliver exceptional flow properties, fast mass transfer, and stability.

Nuvia cPrime delivers value by providing:

  • New and unique selectivity
  • Salt tolerant media
  • Simple method development
  • Large design space for capture and elution of a variety of biotherapeutic proteins
  • High recovery of target protein
  • Mechanical and chemical stability

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技术指标

官能团
Hydrophobic weak cation exchange

Base matrix composition
Macroporous highly crosslinked polymer

Particle size
70 µm ± 10 µm

Dynamic binding capacity
hlgG: >40 mg/ml (at 10% breakthrough, 300 cm/hr)
Lactoferrin: >60 mg/ml

Ligand density
103–143 µeq/ml

Recommended maximum linear flow rate
50–600 cm/hr

Pressure vs. flow performance
Under 2 bar at a flow rate of 600 cm/hr
(20 cm × 20 cm packed bed; 1.17 comp. factor)

pH stability short-term
2–14

pH stability long-term
3–13

化学稳定性
1.0 N NaOH, 1.0 N HCl, 25% HOAc, 8 M urea, 6 M GuHCl, 6 M KSCN, 3 M NaCl, 1% Triton X-100, 2% SDS + 0.25 M NaCl, 20% EtOH, 70% EtOH, 30% IPA

Shipping solution
20% ethanol with 30 mM sodium sulfite

储存条件
20% ethanol

在 20% 乙醇中的保质期
5 years at room temperature

订购

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Nuvia cPrime Media | Bio-Rad Laboratories

(停产) EconoFit Nuvia cPrime Column, 1 x 1 ml

12009281

Pkg of 1, 1 ml, prepacked hydrophobic cation exchange column, 7 mm x 25 mm, max. pressure 45 psi, for use with NGC Systems or any chromatography system

Nuvia cPrime Media | Bio-Rad Laboratories

(停产) Nuvia™ cPrime™ Media

1563401

25 ml, hydrophobic cation exchange chromatography media, 70 µm particle size

Nuvia cPrime Media | Bio-Rad Laboratories

(停产) Foresight™ Nuvia™ cPrime™ Column

7324722

Pkg of 1, 1 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

Nuvia cPrime Media | Bio-Rad Laboratories

(停产) Foresight™ Nuvia™ cPrime™ RoboColumn Unit

7324808

1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

Nuvia cPrime Media | Bio-Rad Laboratories

(停产) Foresight™ Nuvia™ cPrime™ RoboColumn Unit

7324807

1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

Nuvia cPrime Media | Bio-Rad Laboratories

(停产) Foresight™ Nuvia™ cPrime™ Plates

7324705

2 x 96-well plates, 20 μl, filter plate prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

Nuvia cPrime Media | Bio-Rad Laboratories

(停产) Foresight™ Nuvia™ cPrime™ Column

7324742

Pkg of 1, 5 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

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