The SEQuoia Complete Stranded RNA Library Prep Kit is a new RNA-Seq library prep kit with a proprietary enzyme designed to address the sensitivity and workflow limitations of current enzymes.

The SEQuoia Complete Stranded RNA Library Prep Kit is a high-performance RNA-Seq kit that captures long and short RNAs in a single library, even from limited and low-quality samples. The streamlined workflow minimizes the number of pipetting steps and reduces the overall protocol time to less than 4 hours. The kit allows construction of libraries that are >99% stranded and suitable for next-generation sequencing on Illumina platforms and includes access to SEQSense Analysis Solution and Toolkit, a dedicated secondary data analysis pipeline.

Featuring SEQzyme, a proprietary engineered enzyme that couples cDNA synthesis with adapter addition in a continuous synthesis reaction. The unique enzymatic properties effectively captures all types and sizes of RNA species in a novel enzymatic reaction, significantly improving the diversity and quality of RNA libraries, even from limited or degraded RNA samples.

Highly Efficient and Streamlined Library Prep Workflow

The unique enzymatic properties of SEQzyme produce robust, high-quality RNA library. The kits are optimized to facilitate efficient library synthesis using a wide range of input RNA (100 pg to 1 μg) and are compatible with automated next-generation sequencing library construction. The streamlined workflow minimizes the number of pipetting steps and greatly reduces the overall protocol time to less than 4 hours.

Diverse Libraries with Low Quality and Limited Input Samples

The kit constructs a stranded (>99%) library that captures all RNA biotypes, both long and short RNAs, even from poor quality RNAs (for example, FFPE tissue samples). The feature enables researchers to discover biologically relevant differences in more areas of the transcriptome.

Dedicated Data Analysis Toolkit

SEQSense Analysis Solutions and Toolkit, which is available through a web portal, or can be downloaded and run locally, enables users to trim raw sequencing data and align sequences to a reference genome. The output provides specific quality control metrics to assess the quality of the sequencing data as well as a BAM file, which can be used with tertiary analysis packages to perform advanced analyses.

Applications and Uses

• Differential gene expression and whole transcriptome profiling of high- and low-quality RNA samples
  • Sorted or single cell
  • Micro-dissection
  • Characterization of non-polyadenylated RNAs
  • Gene fusion identification
  • Single nucleotide variation discovery
• Biomarker discovery
• Splice junctions identification

Included Components

Each kit comes with 2 reagent boxes. Box A includes reagents for RNA fragmentation, cDNA synthesis, and library preparation. The kit is stored at –20°C:

• Reagent A Fragmentation Mix
• Reagent B End Repair Enzyme
• Reagent C Poly(A) Buffer
• Reagent D Poly(A) Polymerase
• Reagent E SEQzyme Mix
• Reagent F Amplification Mix

Box B includes magnetic bead for purification. The kit is stored at 4°C

SEQuoia Dual Indexed Primers are available and can be ordered separately.

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