G418 (Geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1; blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

98%

Store at -20℃ for one year（Powder）；Store at 2-4℃ for two weeks；Store at -20℃ for six months after dissolution.

Geneticin sulfate; Antibiotic G-418 sulfate

白色粉末

Water：100mg/ml(144.36 mM)

Bacterial

G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3′-phosphotransferase, APT 3′ II is commonly used in laboratory research to select genetically engineered cells. In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks.

Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice.

[1]. Davies J, et al. Am J Trop Med Hyg, 1980, 29(5 Suppl), 1089-1092