Purified Mouse Monoclonal Antibody (Mab)

RNF20抗体;E3 ubiquitin-protein ligase BRE1A; BRE1-A; hBRE1; 632-; RING finger protein 20; RNF20; BRE1A

Mouse

This RNF20 antibody is generated from a mouse immunized with a recombinant protein of human RNF20.

Human

WB~~1:2000

Predicted molecular weight: 114kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Monoclonal Antibody

1594CT552.128.36

IgG1,k

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cell Biology

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of ‘Lys-120’ of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 ‘Lys-4’ and ‘Lys-79’ methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Nucleus

Q5VTR2

Wu H.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zhu B.,et al.Mol. Cell 20:601-611(2005).