Mouse anti-MAFK Monoclonal Antibody(1328CT786.105.125)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Purified Mouse Monoclonal Antibody (Mab)
|
| 别名 |
MAFK抗体;Transcription factor MafK; Erythroid transcription factor NF-E2 p18 subunit; MAFK
|
| 宿主 |
Mouse
|
| 特异性 |
This MAFK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human MAFK.
|
| 反应种属 |
Human
|
| 应用 |
FC~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 18kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
1328CT786.105.125
|
| 同种型 |
IgG1,κ
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 研究领域 |
Crown Antibodies
|
| 靶标 | |
| 背景说明 |
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
|
| 细胞定位 |
Nucleus.
|
| UniProt |
O60675
|
| 参考文献 | |
| 参考文献 |
Toki T.,et al.Oncogene 14:1901-1910(1997).
Hillier L.W.,et al.Nature 424:157-164(2003). Daub H.,et al.Mol. Cell 31:438-448(2008). Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009). |
实验结果图
Flow cytometric analysis of Hela cells using MAFK Antibody(green, Cat#JP100448) compared to an isotype control of mouse IgG1(blue). JP100448 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysate from Jurkat cell line, using MAFK Antibody(Cat. #JP100448). JP100448 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg.