Mouse anti-H2AFX Monoclonal Antibody(C-term)(938CT5.1.1)描述别名宿主特异性反应种属应用分子量类型克隆号同种型储存/保存方法存储溶液研究领域背景说明细胞定位UniProt参考文献
| 概述 | |
| 描述 |
Mouse Monoclonal Antibody (Mab)
|
| 别名 |
H2AFX抗体;Histone H2AX; H2a/x; H2AFX; H2AX
|
| 宿主 |
Mouse
|
| 特异性 |
This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.
|
| 反应种属 |
Human
|
| 应用 |
IHC-P~~1:25
WB~~1:1000 |
| 分子量 |
Predicted molecular weight: 15kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 性能 | |
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
938CT5.1.1
|
| 同种型 |
IgG1
|
| 储存/保存方法 |
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
|
| 存储溶液 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
| 研究领域 |
Crown Antibodies
|
| 靶标 | |
| 背景说明 |
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
|
| 细胞定位 |
Nucleus. Chromosome.
|
| UniProt |
P16104
|
| 参考文献 | |
| 参考文献 |
Stewart G.S., et al. Nature 421:961-966(2003).
Park E.-J., et al. Nucleic Acids Res. 31:6819-6827(2003). Stiff T., et al. Cancer Res. 64:2390-2396(2004). Lukas C., et al. EMBO J. 23:2674-2683(2004). Kurz E.U., et al. J. Biol. Chem. 279:53272-53281(2004). |
实验结果图
Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (C-term)(Cat#JP100345). JP100345 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. thymus section using H2AFX Antibody (C-term)(Cat#JP100345). JP100345 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right). using H2AFX Antibody (C-term)(Cat. #JP100345). JP100345 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.