Rabbit anti-TrkB Recombinant Monoclonal Antibody(S-488-5)描述别名宿主特异性反应种属预测反应种属应用免疫原形式浓度纯化方法类型克隆号储存/保存方法存储溶液背景说明组织特异性翻译后修饰细胞定位UniProt
| 概述 | |
| 描述 |
Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds both BDNF and NT-4 equally well, and binds NT-3 to a lesser extent, while the Trk C receptor only binds NT-3.
|
| 别名 |
BDNF抗体;
BDNF/NT-3 growth factors receptor; GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; NTRK2 |
| 宿主 |
Rabbit
|
| 特异性 |
BDNF Antibody detects endogenous levels of total BDNF.
|
| 反应种属 |
Human, Mouse, Rat
|
| 预测反应种属 |
Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
|
| 应用 |
WB: 1:1000, IHC-P: 1:500
|
| 免疫原 |
Recombinant Protein
|
| 性能 | |
| 形式 |
Liquid
|
| 浓度 |
0.5 mg/mL
|
| 纯化方法 |
Protein A affinity column
|
| 类型 |
Monoclonal Antibody
|
| 克隆号 |
S-488-5
|
| 储存/保存方法 |
Store at -20℃ for one year.
|
| 存储溶液 |
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
|
| 靶标 | |
| 背景说明 |
Trk receptors are a family of tyrosine kinases that regulates synaptic strength and plasticity in the mammalian nervous system. Trk receptors affect neuronal survival and differentiation through several signaling cascades. The common ligands of trk receptors are neurotrophins, a family of growth factors critical to the functioning of the nervous system. The binding of these molecules is highly specific. Each type of neurotrophin has different binding affinity toward its corresponding Trk receptor. The activation of Trk receptors by neurotrophin binding may lead to activation of signal cascades resulting in promoting survival and other functional regulation of cells.
|
| 组织特异性 |
Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
|
| 翻译后修饰 |
The propeptide is N-glycosylated and glycosulfated.Converted into mature BDNF by plasmin (PLG).
|
| 细胞定位 |
Cell membrane, Cytoplasm
|
| UniProt |
Q16620
|
实验结果图
WB result of TrkB Rabbit mAb Primary antibody: TrkB Rabbit mAb at 1/1000 dilution Lane 1: mouse liver lysate 20 ug Lane 2: mouse brain lysate 20 ug Negative control: mouse liver lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa Observed MW: 140, 90 kDa Exposure time: 90 s
WB result of TrkB Rabbit mAb Primary antibody: TrkB Rabbit mAb at 1/1000 dilution Lane 1: rat liver lysate 20 ug Lane 2: rat brain lysate 20 ug Negative control: rat liver lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 92 kDa Observed MW: 140, 90 kDa Exposure time: 90 s
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-TrkB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human astrocytoma. Anti-TrkB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-TrkB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-TrkB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-TrkB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-TrkB antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.