MTT噻唑蓝 细胞检测M8180

MTT噻唑蓝 细胞检测M8180

产品名称:MTT噻唑蓝 细胞检测

产品型号:M8180

产品特点:MTT噻唑蓝 细胞检测
货号:M8180
规格:250mg/支
CAS:298-93-1
分子式:C18H16N5SBr
分子量:414.3
储存条件:2-8℃ 避光
纯度:>98.0
外观(性状):黄色粉末
单位:瓶

M8180MTT噻唑蓝 细胞检测的详细资料:

MTT噻唑蓝 细胞检测
货号:M8180
规格:250mg/支

  • 别名:3-(4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑溴化物; 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide
  • 英文名称:Thiazolyl blue tetrazolium bromide
  • CAS:298-93-1
  • 分子式:C18H16N5SBr
  • 分子量:414.3
  • 储存条件:2-8℃ 避光
  • 纯度:>98.0
  • 外观(性状):黄色粉末
  • 单位:瓶

说明:

    生化研究中酶活力的测定,也可用于细胞增殖检测。

特性:纯度(%):>98.0 溶解性:溶于甲醇和DMSO,微溶于水

 MTT是一种检测细胞存活和生长的方法。MTT实验原理为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲瓒(Formazan)并沉积在细胞中,而死细胞无此功能。二甲基亚砜(DMSO)能溶解细胞中的甲瓒,用酶联免疫检测仪在490nm波长处测定其光吸收值,可间接反映活细胞数量。
在一定细胞数范围内,MTT结晶形成的量与细胞数成正比。根据测得的吸光度值(OD值),来判断活细胞数量,OD值越大,细胞活性越强(如果是测药物毒性,则表示药物毒性越小)。该方法已广泛用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞毒性试验以及肿瘤放射敏感性测定等。它的特点是灵敏度高、经济。
MTT通常用于.细胞增殖及细胞活性测定以及药物(也包括其他处理方式如放射线照射)对体外培养的细胞毒性的测定。
缺点:由于MTT经还原所产生的甲瓒产物不溶于水,需被溶解后才能检测。这不仅使工作量增加,也会对实验结果的准确性产生影响,而且溶解甲的有机溶剂对实验者也有损害。

MTT噻唑蓝 细胞检测订购说明:

1、绝大部分产品备有现货,一般情况下都能订货当日发货。 
2、部分非常用产品,需提前1-2日预订,海外期货则需要提前3-6周预订。 
3、部分产品价格会因货期、批次等因素发生变化,若有变动以订货当日新价格为准。 
4、每日订单截止时间为16点整,部分城市可到17点整,因超过截止时间造成当日不能发货的将于次日安排发货。
5、请办理完货款后,将收据、底单等连同收货人的地址、姓名、等以传真、、短信、等形式通知我们。

运输说明:

1、极低温产品:极低温产品运输过程中加装干冰运输。用干冰把产品包裹起来,再用泡沫盒密封,胶带层层粘住泡沫盒,放入金畔生物的箱子,然后交付给合作快递,安全快速高效放心的送客户的手中,保证产品的性质稳定如一,为您的实验保驾护航。
2、低温产品:低温产品运输过程中加装金畔生物冰袋运输。事先用冰袋把产品包裹起来,再使用泡沫盒密封,用胶带严实密封泡沫盒,再放入金畔生物的箱子(保温效果少可以持续一周),然后交付给合作快递,安全快速高效放心的送客户的手中,保证产品的性质稳定如一,为您的实验保驾护航。
3、常温产品:常温产品运输过程中无需加冰或者特殊包装。产品由公司库房人员快速配货,准确快速高效的快递保证产品快速送达您的手中。

 

 

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